Recombinant
RabMAb

Anti-Insulin antibody [EPR17359] (ab181547)

Overview

  • Product name
    Anti-Insulin antibody [EPR17359]
    See all Insulin primary antibodies
  • Description
    Rabbit monoclonal [EPR17359] to Insulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Insulin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01308

  • Positive control
    • IHC-P: Human pancreas, Mouse pancreas and Rat pancreas tissues. IHC-FoFr: Mouse pancreas tissue. ICC/IF: BxPC-3 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.
IHC-P 1/64000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/200.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
    • Involvement in disease
      Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
      Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
      Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities
      Belongs to the insulin family.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    Images

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Insulin with ab181547 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows cytoplasmic staining on BxPC-3 cells. The nuclear counter stain is Dapi (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1: - ab181547 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of Human pancreas is observed. Counter stained with Hematoxylin.

      Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Negative staining on Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Negative staining on Human liver tissue is observed. Counter stained with Hematoxylin.

    • Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counter stained with Hematoxylin.

      Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    • Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of rat pancreas is observed. Counter stained with Hematoxylin.

      Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.

    • Immunohistochemical analysis of 4% paraformaldehyde perfusion fixed, frozen section of Mouse pancreas tissue labeling Insulin with ab181547 at 1/1000 dilution, followed by Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counter stained with DAPI.

      Negative control: PBS instead of primary antibody; secondary antibody is Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution.

    References

    This product has been referenced in:
    • Kehm R  et al. Age-related oxidative changes in pancreatic islets are predominantly located in the vascular system. Redox Biol 15:387-393 (2018). Read more (PubMed: 29331666) »
    • Yu H  et al. The rat pancreatic body tail as a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering. J Biol Eng 12:6 (2018). Read more (PubMed: 29719565) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 31 2018

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (pancreas)
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 22 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrat-Buffer pH 6,0
    Permeabilization
    No
    Specification
    pancreas
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 17 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA PH 9
    Specification
    Pancreas
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde
    Username

    Dr. Toloo Taghian

    Verified customer

    Submitted Apr 22 2016

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Tissue sections (pancreas)
    Specification
    pancreas
    Permeabilization
    Yes - 0.5% Triton X100
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 31 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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