Anti-Insulin antibody [EPR17359] (ab181547)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-Insulin antibody [EPR17359]
See all Insulin primary antibodies -
DescriptionRabbit monoclonal [EPR17359] to Insulin
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, IHC-P, ICC/IFmore details
Unsuitable for: WB -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human Insulin aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P01308 -
Positive control
- IHC-P: Human pancreas, Mouse pancreas and Rat pancreas tissues. IHC-FoFr: Mouse pancreas tissue. ICC/IF: BxPC-3 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR17359
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab181547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | 1/1000. | |
| IHC-P | 1/64000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| ICC/IF | 1/200. |
Target
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FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similaritiesBelongs to the insulin family.
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Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3630 Human
- Entrez Gene: 16333 Mouse
- Entrez Gene: 24505 Rat
- Omim: 176730 Human
- SwissProt: P01308 Human
- SwissProt: P01325 Mouse
- SwissProt: P01322 Rat
- Unigene: 272259 Human
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Alternative names
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-244698-ab.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Insulin antibody [EPR17359] (ab181547)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling Insulin with ab181547 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows cytoplasmic staining on BxPC-3 cells. The nuclear counter stain is Dapi (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: - ab181547 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232403-InsulinIHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of Human pancreas is observed. Counter stained with Hematoxylin.
Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232404-InsulinIHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Negative staining on Human colonic adenocarcinoma is observed. Counter stained with Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232405-InsulinIHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of paraffin-embedded Human liver tissue with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Negative staining on Human liver tissue is observed. Counter stained with Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232406-InsulinIHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counter stained with Hematoxylin.
Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232407-InsulinIHC5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Insulin with ab181547 at 1/64000 dilution, followed by Anti-Rabbit HRP (ab97051) at 1/500 dilution. Cytoplasm staining on islet cells of rat pancreas is observed. Counter stained with Hematoxylin.
Negative control: PBS instead of primary antibody; secondary antibody is Anti-Rabbit HRP (ab97051) at 1/500 dilution.
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![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin antibody [EPR17359] (ab181547)](https://www.abcam.com/ps/products/181/ab181547/Images/ab181547-232410-IHCF.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin antibody [EPR17359] (ab181547)Immunohistochemical analysis of 4% paraformaldehyde perfusion fixed, frozen section of Mouse pancreas tissue labeling Insulin with ab181547 at 1/1000 dilution, followed by Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution. Cytoplasm staining on islet cells of mouse pancreas is observed. Counter stained with DAPI.
Negative control: PBS instead of primary antibody; secondary antibody is Donkey anti-rabbit Alexa Fluor 594 at 1/1000 dilution.
References
This product has been referenced in:
- Kehm R et al. Age-related oxidative changes in pancreatic islets are predominantly located in the vascular system. Redox Biol 15:387-393 (2018). Read more (PubMed: 29331666) »
- Yu H et al. The rat pancreatic body tail as a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering. J Biol Eng 12:6 (2018). Read more (PubMed: 29719565) »
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