Overview

  • Product name

    Anti-Insulin antibody [EPR3075]
    See all Insulin primary antibodies
  • Description

    Rabbit monoclonal [EPR3075] to Insulin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Insulin aa 50 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Human fetal pancreas lysate; Human pancreas tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
    • Involvement in disease

      Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
      Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
      Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities

      Belongs to the insulin family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    Images

    • Anti-Insulin antibody [EPR3075] (ab108326) at 1/1000 dilution + Human fetal pancreas lysate at 10 µg

      Predicted band size: 12 kDa

    • ab108326 at 1/100 dilution staining insulin in Human pancreas by Immunohistochemistry, Paraffin-embedded tissue.

    References

    ab108326 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Gracias por escribirnos.

    Si lo que quiere la clienta es un anticuerpo contra C peptide para ICC que reaccione en humano, existen varias opciones.

    Si lo que requiere además es que no reaccione con rata ni ratón me temo que las opciones se reducen a ab108326. Lamentablemente no hay información acerca de la posibilidad de ab8297 de reconocer la proteína en rata/ratón, puesto que nunca se ha testado en nuestro laboratorio.

    Lo que puedo sugerirle a la clienta es que pruebe ab108326 en ICC y participe en nuestro sistema de evaluación de anticuerpos Abreview.

    Si le interesa, puede usar el anticuerpo en esta nueva aplicación no testada hasta ahora (y que es muy posible que funcione), y nos envíe sus resultados a la web en forma de Abreview. Por el hecho de compartirlos con nosotros se beneficiará de un próximo anticuerpo completamente gratuito.

    Si le interesara esta opción, o requerís mas información acerca de los Abreviews, por favor, no dudes en contactarme, que como siempre estaré encantada de poder ayudar.

    Espero pues tu respuesta. Que pases un gran día.

    Read More

    Answer

    Thank you for your inquiry.

    For ab8297, we have never tested this antibody for cross-reactivity with mouse or rat. Therefore, we would not be able to guarantee it for non-cross-reactivity with mouse and rat.

    For ab108326, this antibody does not show cross-reactivity with mouse or rat by WB analysis. However, we wouldn't be able to guarantee this antibody in ICC/IF, since we didn't get good results in this application. We would, however, guarantee it to work in IHC-P, so this seems like the closest match to what you're looking for, although not perfect.

    I hope this information helps. Please contact us with any other questions.

    Read More

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