Recombinant
RabMAb

Recombinant Anti-Insulin antibody [EPR3075] - BSA and Azide free (ab221801)

Overview

  • Product name

    Anti-Insulin antibody [EPR3075] - BSA and Azide free
    See all Insulin primary antibodies
  • Description

    Rabbit monoclonal [EPR3075] to Insulin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Insulin aa 50 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Human fetal pancreas lysate; Human pancreas tissue.
  • General notes

    ab221801 is the carrier-free version of ab108326 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab221801 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
IHC-P Use at an assay dependent concentration.

Perform antigen retrieval.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
    • Involvement in disease

      Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
      Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
      Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
      Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    • Sequence similarities

      Belongs to the insulin family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • IDDM antibody
      • IDDM1 antibody
      • IDDM2 antibody
      • ILPR antibody
      • ins antibody
      • INS_HUMAN antibody
      • Insulin A chain antibody
      • Insulin B chain antibody
      • IRDN antibody
      • MODY10 antibody
      • Preproinsulin antibody
      • Proinsulin antibody
      • Proinsulin precursor antibody
      see all

    References

    ab221801 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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