Overview

  • Product name

    Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free
    See all Insulin primary antibodies
  • Description

    Mouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free
  • Host species

    Mouse
  • Specificity

    The antibody blocks binding of insulin to the receptor.
  • Tested applications

    Suitable for: ELISA, RIA, ICC/IF, IHC-P, Functional Studiesmore details
  • Species reactivity

    Reacts with: Cow, Human, Pig
  • Immunogen

    Porcine insulin

  • Positive control

    • IHC-P: Human pancreas tissue sections.
  • General notes

    Preserved by filter sterilization

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab46707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Blocks the binding of insulin to the receptor.

Target

  • Function

    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • Involvement in disease

    Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities

    Belongs to the insulin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Images

  • IHC image of Insulin staining in human pancreas formalin fixed paraffin embedded tissue section (ab4611), performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46707, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Ren R  et al. Nanopore extended field-effect transistor for selective single-molecule biosensing. Nat Commun 8:586 (2017). Read more (PubMed: 28928405) »
  • Woods CA  et al. Insulin receptor activation in the nucleus accumbens reflects nutritive value of a recently ingested meal. Physiol Behav 159:52-63 (2016). Read more (PubMed: 26988281) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your reply.

While we have a number of anti-Insulin antibodies which you would be able to conjugated to an organic fluorophore, we currently do not offer AlexaFluor 500 in our EasyLink conjugation kits. We do offer a number of other fluorophores including FITC which have similar excitation and emission wavelengths. More about our EasyLink conjugation kits and the fluorescent labels available may be found at:

https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

Although we have many anti-Insulin antibodies, when using a conjugation kit you will want to make sure the antibody which you will be conjugating to is purified and has a concentration of at least 0.5mg/ml. Two such products are ab46707 and ab63820.

https://www.abcam.com/insulin-antibody-in-05-azide-free-ab46707.html

https://www.abcam.com/insulin-antibody-in-05-azide-free-ab46707.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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