Key features and details
- Mouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free
- Suitable for: IHC-P
- Reacts with: Cow, Human, Pig
- Isotype: IgG1
Product nameAnti-Insulin antibody [IN-05] - Low endotoxin, Azide free
See all Insulin primary antibodies
DescriptionMouse monoclonal [IN-05] to Insulin - Low endotoxin, Azide free
SpecificityThe antibody blocks binding of insulin to the receptor.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Cow, Human, Pig
- IHC-P: Human pancreas tissue sections.
Preserved by filter sterilization
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab46707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the insulin family.
- Information by UniProt
- IDDM antibody
- IDDM1 antibody
- IDDM2 antibody
IHC image of Insulin staining in human pancreas formalin fixed paraffin embedded tissue section (ab4611), performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46707, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab46707 has been referenced in 4 publications.
- Ren R et al. Nanopore extended field-effect transistor for selective single-molecule biosensing. Nat Commun 8:586 (2017). PubMed: 28928405
- Woods CA et al. Insulin receptor activation in the nucleus accumbens reflects nutritive value of a recently ingested meal. Physiol Behav 159:52-63 (2016). PubMed: 26988281
- Stouffer MA et al. Insulin enhances striatal dopamine release by activating cholinergic interneurons and thereby signals reward. Nat Commun 6:8543 (2015). PubMed: 26503322
- Okere B et al. In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids. Int J Immunopathol Pharmacol 28:390-402 (2015). PubMed: 26216908