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Indeed, We are trying to build an insulin biosensor based on the detection of a fluorescence signal. To do so, I would like to have an organic fluorophore (Alexa Fluor 500) attached to the antibody. The cystein group I mentioned before will be used to attach the fluorecent antibody on the biosensor surface using the malemide chemistry. So I would like to have : Cystein-Anti-insulin-Alexa Fluor 500
Asked on Nov 06 2012
Thank you for your reply.
While we have a number of anti-Insulin antibodies which you would be able to conjugated to an organic fluorophore, we currently do not offer AlexaFluor 500 in our EasyLink conjugation kits. We do offer a number of other fluorophores including FITC which have similar excitation and emission wavelengths. More about our EasyLink conjugation kits and the fluorescent labels available may be found at:
Although we have many anti-Insulin antibodies, when using a conjugation kit you will want to make sure the antibody which you will be conjugating to is purified and has a concentration of at least 0.5mg/ml. Two such products are ab46707 and ab63820.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 06 2012