Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description

    Rabbit polyclonal to Insulin degrading enzyme / IDE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Insulin degrading enzyme/ IDE (internal sequence). The exact sequence is proprietary.
    Database link: P14735

  • Positive control

    • WB: HeLa cell extract; Mouse and rat brain extracts; A431, NCI-H1299, HeLa and HepG2 whole cell lysates. ICC/IF: HeLa cells. IHC-P: U87 xenograft tissue; Rat brain stem tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 118 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution

    Lane 1 : WT HeLa cell extract
    Lane 2 : Insulin degrading enzyme / IDE Knockout HeLa cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 118 kDa

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution

    Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 2 : Insulin degrading enzyme / IDE knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 118 kDa



    7.5% SDS-PAGE gel.

  • Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Insulin degrading enzyme / IDE (green) using ab228720 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded U87 xenograft tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/1000 dilution

    Lane 1 : Mouse brain extract
    Lane 2 : Rat brain extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 118 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/10000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 118 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded rat brain stem tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.

References

ab228720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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