Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description

    Rabbit polyclonal to Insulin degrading enzyme / IDE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 275-326. The exact sequence is proprietary. NP_004960.1
    Database link: P14735

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab245521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 118 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
    • Sequence similarities

      Belongs to the peptidase M16 family.
    • Post-translational
      modifications

      The N-terminus is blocked.
    • Cellular localization

      Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
    • Information by UniProt
    • Database links

    • Alternative names

      • Abeta-degrading protease antibody
      • FLJ35968 antibody
      • Ide antibody
      • IDE_HUMAN antibody
      • Insulin protease antibody
      • Insulin-degrading enzyme antibody
      • Insulinase antibody
      • Insulysin antibody
      • OTTHUMP00000020097 antibody
      see all

    Images

    • All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab245521) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 118 kDa


      Exposure time: 3 minutes

    References

    ab245521 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245521.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up