Product nameAnti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies
DescriptionRabbit polyclonal to Insulin degrading enzyme / IDE
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 275-326. The exact sequence is proprietary. NP_004960.1
Database link: P14735
- WB: HeLa and HEK-293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245521 was affinity purified using an epitope specific to Insulin degrading enzyme / IDE immobilized on solid support.
Our Abpromise guarantee covers the use of ab245521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 118 kDa.|
FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similaritiesBelongs to the peptidase M16 family.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
- Information by UniProt
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab245521) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 3 minutes
ab245521 has not yet been referenced specifically in any publications.