Product nameAnti-Insulin degrading enzyme / IDE antibody [EPR6098(2)]
See all Insulin degrading enzyme / IDE primary antibodies
DescriptionRabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 50-150. The exact sequence is proprietary.
Database link: P14735
- HeLa, HepG2, A375, and K562 cell lysates, Human colon tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.31 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 118 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similaritiesBelongs to the peptidase M16 family.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
- Information by UniProt
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Cai H et al. The Mechanisms of Bushen-Yizhi Formula as a Therapeutic Agent against Alzheimer's Disease. Sci Rep 8:3104 (2018). Read more (PubMed: 29449587) »
- Huang Y et al. Chinese Herbal Medicine Xueshuantong Enhances Cerebral Blood Flow and Improves Neural Functions in Alzheimer's Disease Mice. J Alzheimers Dis 63:1089-1107 (2018). Read more (PubMed: 29710701) »