Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

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Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free
    See all Insulin degrading enzyme / IDE primary antibodies

  • Description

    Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 50-150. The exact sequence is proprietary.
    Database link: P14735

  • Positive control

    • WB: HepG2 and K562 cell lysates. Wild-type HAP1 cell lysate.

  • General notes

    Ab233020 is the carrier-free version of ab133561. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab233020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab233020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 118 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.

  • Sequence similarities

    Belongs to the peptidase M16 family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: IDE knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Other - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Other - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free (ab233020)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133561).

References

ab233020 has not yet been referenced specifically in any publications.

Publishing research using ab233020? Please let us know so that we can cite the reference in this datasheet.

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