Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (HRP) (ab201836)

Overview

  • Product name

    Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (HRP)
    See all Insulin degrading enzyme / IDE primary antibodies
  • Description

    Rabbit monoclonal [EPR6099] to Insulin degrading enzyme / IDE (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 950-1050 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HepG2, A375, and K562 whole cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

Target

  • Function

    Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • Sequence similarities

    Belongs to the peptidase M16 family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt
  • Database links

  • Alternative names

    • Abeta-degrading protease antibody
    • FLJ35968 antibody
    • Ide antibody
    • IDE_HUMAN antibody
    • Insulin protease antibody
    • Insulin-degrading enzyme antibody
    • Insulinase antibody
    • Insulysin antibody
    • OTTHUMP00000020097 antibody
    see all

Images

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (HRP) (ab201836) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : IDE (Insulin degrading enzyme / IDE) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa


    Exposure time: 20 minutes


    ab201836 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells as signal was lost in IDE (Insulin degrading enzyme / IDE) knockout cells. Wild-type and IDE (Insulin degrading enzyme / IDE) knockout samples were subjected to SDS-PAGE. Ab201836 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6099] (HRP) (ab201836) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : A375 (Human melanoma cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 118 kDa
    Observed band size: 118 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab201836 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab201836 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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