Product nameAnti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies
DescriptionRabbit polyclonal to Insulin degrading enzyme / IDE
Tested applicationsSuitable for: WB, IHC-P, IHC-FoFr, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Dog
- Mouse and Rat brain lysate. Mouse and Rat hippocampus lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab32216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Insulin degrading enzyme / IDE peptide (ab32215).|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
Sequence similaritiesBelongs to the peptidase M16 family.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
- Information by UniProt
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
Image courtesy of Human Protein Atlas
ab32216 staining human Gall bladder. The paraffin embedded human skin tissue was incubated with ab32216 (1/50 dilution) for 30 mins at room temperature. The staining is predominantly cytoplasmic although some nuclear staining can be seen. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab32216 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for
All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml
Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
This product has been referenced in:
- Kulas JA et al. Ablation of amyloid precursor protein increases insulin-degrading enzyme levels and activity in brain and peripheral tissues. Am J Physiol Endocrinol Metab 316:E106-E120 (2019). Read more (PubMed: 30422705) »
- Fu W et al. Tongluo Xingnao effervescent tablet reverses memory deficit and reduces plaque load in APPswe/PS1dE9 mice. Exp Ther Med 15:4005-4013 (2018). Read more (PubMed: 29563991) »