Recombinant Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)
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Overview
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Product nameAnti-Insulin Receptor beta antibody [EPR22167]
See all Insulin Receptor beta primary antibodies -
DescriptionRabbit monoclonal [EPR22167] to Insulin Receptor beta
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human Insulin Receptor beta aa 950 to the C-terminus. The exact sequence is proprietary.
Database link: P06213 -
Positive control
- WB: HepG2, HEK-293T, LNCaP, HeLa and MCF7 whole cell lysates; Human fetal brain and liver lysates. IHC-P: Human kidney and kidney carcinoma tissues. IP: HepG2 whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR22167
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 210, 95, 49 kDa (predicted molecular weight: 156 kDa). | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | 1/30. |
Target
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RelevanceInsulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
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Cellular localizationMembrane; Single pass type I membrane protein.
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Database links
- Entrez Gene: 3643 Human
- Omim: 147670 Human
- SwissProt: P06213 Human
- Unigene: 438669 Human
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Alternative names
- CD220 antibody
- HHF5 antibody
- HIR B antibody
see all
Images
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![Western blot - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)](https://www.abcam.com/ps/products/227/ab227831/Images/ab227831-313599-227831WB.jpg)
Western blot - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)All lanes : Anti-Insulin Receptor beta antibody [EPR22167] (ab227831) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 3 : LNCaP (human prostate cancer cell line) whole cell lysate at 10 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 6 : Human fetal brain lysate at 10 µg
Lane 7 : Human liver lysate at 10 µg
Secondary
Lanes 1-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 6-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 156 kDaExposure time : Lanes 1-2: 48 seconds; Lanes 3: 59 seconds; Lanes 4&6-7: 70 seconds; Lane 5: 37 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The 210 kDa band is the pro-insulin receptor, while the 95 kDa band is the insulin receptor beta subunit (PMID: 28765322, PMID: 28915606).
The 45-68 kDa bands are proteolytic cleavage fragments (PMID: 28915606, PMID: 6693383, PMID: 6315728).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)](https://www.abcam.com/ps/products/227/ab227831/Images/ab227831-313595-227831IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)Immunohistochemical analysis of paraffin-embedded human kidney (Panel A) and kidney carcinoma (Panel B) tissues labeling Insulin Receptor beta with ab227831 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human kidney tubules (panel A). Positive staining in endothelium of blood vessels in human kidney carcinoma (panel B), PMID: 25864925, PMID: 20182859. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)](https://www.abcam.com/ps/products/227/ab227831/Images/ab227831-313645-831b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Insulin Receptor beta with ab227831 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in endothelium of blood vessels in human glioma (PMID: 26136493) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
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![Immunoprecipitation - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)](https://www.abcam.com/ps/products/227/ab227831/Images/ab227831-313594-227831IP.jpg)
Immunoprecipitation - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)Insulin Receptor beta was immunoprecipitated from 0.35 mg of HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab227831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HepG2 whole cell lysate 10 µg (Input).
Lane 2: 227831 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of 227831 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The 210 kDa band is the pro-Insulin receptor, while the 95 kDa band is the insulin receptor beta subunit (PMID: 28765322, PMID: 28915606).
The 45-68 kDa bands are proteolytic cleavage fragments (PMID: 28915606, PMID: 6693383, PMID: 6315728).
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![Immunoprecipitation - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)](https://www.abcam.com/ps/products/227/ab227831/Images/ab227831-313644-831.jpg)
Immunoprecipitation - Anti-Insulin Receptor beta antibody [EPR22167] (ab227831)Insulin Receptor beta was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab227831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227831 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: HEK-293T whole cell lysate 10 µg (Input).
Lane 2: 227831 IP in HEK-293T whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of 227831 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The 210 kDa band is the pro-Insulin receptor, while the 95 kDa band is the insulin receptor beta subunit (PMID: 28765322, PMID: 28915606).
The 45-68 kDa bands are proteolytic cleavage fragments (PMID: 28915606, PMID: 6693383, PMID: 6315728).
Datasheets and documents
References
ab227831 has not yet been referenced specifically in any publications.
