Overview

  • Product name

    Anti-Insulin Receptor beta (phospho Y1185) antibody
    See all Insulin Receptor beta primary antibodies
  • Description

    Rabbit polyclonal to Insulin Receptor beta (phospho Y1185)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Insulin Receptor beta aa 1160-1200 (phospho Y1185) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    L AARNCMVAHD FTVKIGDFGM TRDIYETDYY RKGGKGLLPV


    Database link: P06213

  • Positive control

    • Human lung tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab203278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 156 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Relevance

    Insulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
  • Cellular localization

    Membrane; Single pass type I membrane protein.
  • Database links

  • Alternative names

    • CD220 antibody
    • HHF5 antibody
    • HIR B antibody
    • INSR antibody
    • Insulin receptor antibody
    • Insulin receptor subunit beta antibody
    • IR antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human lung tissue labeling Insulin Receptor beta (phospho Y1185) using ab203278 at 1/200, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded tissue labeling Insulin Receptor beta (phospho Y1185) using ab203278 at 1/200, followed by secondary detection and DAB staining.

References

ab203278 has not yet been referenced specifically in any publications.

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