Key features and details
- Rabbit polyclonal to Insulin Receptor beta (phospho Y1185)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Insulin Receptor beta (phospho Y1185) antibody
See all Insulin Receptor beta primary antibodies
DescriptionRabbit polyclonal to Insulin Receptor beta (phospho Y1185)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human lung tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 156 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
RelevanceInsulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc.
Cellular localizationMembrane; Single pass type I membrane protein.
- CD220 antibody
- HHF5 antibody
- HIR B antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded human lung tissue labeling Insulin Receptor beta (phospho Y1185) using ab203278 at 1/200, followed by secondary detection and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin embedded tissue labeling Insulin Receptor beta (phospho Y1185) using ab203278 at 1/200, followed by secondary detection and DAB staining.
ab203278 has not yet been referenced specifically in any publications.