INT dye, tetrazolium dye precursor (ab146338)

Overview

  • Product name

    INT dye, tetrazolium dye precursor
  • Description

    Tetrazolium dye precursor used to measure dehydrogenase activity.
  • Alternative names

    • 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride
    • INT
    • Iodonitrotetrazolium chloride
    • Iodonitrotetrazolium purple
    • Iodonitrotetrazolum violet
    • p-Iodonitrotetrazolium Violet
    see all
  • Biological description

    Tetrazolium dye precursor that forms a purple formazan dye on reduction. Used as an electron acceptor for the colorimetric assay of dehydrogenase activity. Also used for western blot staining and as a staining agent for soft agar colony formation assays.

  • Purity

    > 98%
  • CAS Number

    146-68-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride
  • Molecular weight

    505.71
  • Molecular formula

    C19H13ClIN5O2
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Berridge MV  et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev 11:127-52 (2005). Read more (PubMed: 16216776) »
  • Green JD & Narahara HT Assay of succinate dehydrogenase activity by the tetrazolium method: evaluation of an improved technique in skeletal muscle fractions. J Histochem Cytochem 28:408-12 (1980). Read more (PubMed: 6966645) »

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