Product nameAnti-INT4 antibody
See all INT4 primary antibodies
DescriptionRabbit polyclonal to INT4
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to Human INT4 aa 913-963.
Database link: NP_291025.3
- HeLa, 293T and NIH3T3 whole cell lysates.
Previously labelled as INTS4.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75253 was affinity purified using an epitope specific to INT4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab75253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 108 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.
Sequence similaritiesBelongs to the Integrator subunit 4 family.
Contains 8 HEAT repeats.
- Information by UniProt
- Int4 antibody
- INT4_HUMAN antibody
- Integrator complex subunit 4 antibody
All lanes : Anti-INT4 antibody (ab75253) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling INT4 with ab75253 at 1/1000 (1µg/ml). Detection: DAB.
Detection of INT4 by Western Blot of Immunprecipitate.
ab75253, at 1µg/ml, staining INT4 in HeLa whole cell lysate immunoprecipitated using ab75253 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.