Anti-Integrin alpha 1 antibody (ab200570)
Key features and details
- Goat polyclonal to Integrin alpha 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin alpha 1 antibody
See all Integrin alpha 1 primary antibodies -
Description
Goat polyclonal to Integrin alpha 1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Integrin alpha 1 aa 765-776 (internal sequence) (Cysteine residue). NP_852478.1.
Sequence:DKHDFQDSVRIT
Database link: P56199 -
Positive control
- HeLa and HepG2 cell lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3672 Human
- Omim: 192968 Human
- SwissProt: P56199 Human
- Unigene: 644352 Human
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Alternative names
- CD 49a antibody
- CD49 antigen-like family member A antibody
- CD49a antibody
see all
Images
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Western blot - Anti-Integrin alpha 1 antibody (ab200570)All lanes : Anti-Integrin alpha 1 antibody (ab200570) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (0)
ab200570 has not yet been referenced specifically in any publications.
