Anti-Integrin alpha 1 antibody (ab200570)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin alpha 1 antibody
See all Integrin alpha 1 primary antibodies -
Description
Goat polyclonal to Integrin alpha 1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Integrin alpha 1 aa 765-776 (internal sequence) (Cysteine residue). NP_852478.1.
Sequence:DKHDFQDSVRIT
Database link: P56199 -
Positive control
- HeLa and HepG2 cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 130 kDa). |
Target
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Function
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3672 Human
- Omim: 192968 Human
- SwissProt: P56199 Human
- Unigene: 644352 Human
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Alternative names
- CD 49a antibody
- CD49 antigen-like family member A antibody
- CD49a antibody
see all
Images
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Western blot - Anti-Integrin alpha 1 antibody (ab200570)All lanes : Anti-Integrin alpha 1 antibody (ab200570) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 130 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab200570 has not yet been referenced specifically in any publications.
