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Rabbit polyclonal to Integrin alpha 1
ab78479 is specific to Integrin alpha 1. No cross reactivity to alpha2, alpha3, alpha4, alpha5, alpha6, or alphaV integrin subunits.
ICC/IF more details
Rat, Sheep, Goat, Horse, Chicken, Hamster, Human, Pig, Monkey
Synthetic peptide corresponding to Rat Integrin alpha 1 aa 1166-1180 (C terminal). Cytoplasmic domain.
PC12 cells. Rhesus monkey maternal uterine endometrium tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: Whole serum
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 10
1/100 - 1/1000. Detects a band of approximately 200 kDa (predicted molecular weight: 131 kDa).
Use at an assay dependent dilution.
1/200. Suggested for use on acetone fixed tissue only. This antibody has been reported to react in 4% PFA fixed monkey tissue treated with citrate.
Use at an assay dependent concentration.
Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen.
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Information by UniProt
CD 49a antibody
CD49 antigen-like family member A antibody
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 1 antibody - Cytoplasmic domain (ab78479)
ICC/IF image of ab78479 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78479, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"