Anti-Integrin alpha 2 antibody [DX5] (Allophycocyanin/Cy7 ®) (ab46795)


  • Product name
    Anti-Integrin alpha 2 antibody [DX5] (Allophycocyanin/Cy7 ®)
    See all Integrin alpha 2 primary antibodies
  • Description
    Rat monoclonal [DX5] to Integrin alpha 2 (Allophycocyanin/Cy7 ®)
  • Host species
  • Conjugation
    Allophycocyanin/Cy7 ®. Ex: 650nm, Em: 774nm
  • Specificity
    ab46795 reacts with mouse CD49b, Mr 160-kDa.
  • Tested applications
    Suitable for: Flow Cytmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    RIL 2 propagated NK1.1+ cells from C57BL/6 mice. (Fusion Partner Mouse SP2/0 myeloma cell line).

  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.



Our Abpromise guarantee covers the use of ab46795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µl for 106 cells.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Function
      Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • Domain
      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha II antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all


    • FACS analysis of B6 splenocytes, doublestained with rat anti-mouse CD49b (integrin alpha 2)-APC/Cy7 (ab46795) at 0.2 µl/106 cells and CD3-FITC.


    ab46795 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for your e-mail.
    I have contacted the Lab which produce this antibody regarding your question:
    "The immunogen used to produce this antibody was RIL-2-propagated NK1.1+ cells from C57BL/6 mice, though this antibody stains the majority of NK cells in numerous mouse strains including Balb/c. I have attached the technical bulletin for your reference. "
    From the refference:" The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.
    I hope this helps.Please let us know if you have any other questions.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Flow Cytometry
    Mouse Cell (whole blood)
    whole blood
    Gating Strategy
    monocyte, lymphocyte and granulocyte

    Abcam user community

    Verified customer

    Submitted May 01 2012


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