Name: Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
Brand: RabMAb
SKU: ab181548
Rating: 5 (4 reviews)

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Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal
Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Carrier Free HRP PE Unconjugated

Product name

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal
See all Integrin alpha 2 primary antibodies
Description

Rabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, IP, IHC-P, WB, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A549, A431, 293T,T-47D, C6 and NIH/3T3 whole cell lysates, human fetal brain and fetal heart, mouse heart and kidney, and rat spleen tissue lysates. IHC-P: Human colon, human squamous cell carcinoma of cervix, mouse kidney and rat colon tissues. ICC/IF: Wild-type HAP1, PC-3 and MCF7 cells. Flow Cyt (intra): A549 cells. IP: T-47D whole cell extract.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17338
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		Use a concentration of 1 µg/ml. This product gave a positive signal in wild-type HAP1 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
IP		1/150.
IHC-P	(2)	1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 129 kDa).
Flow Cyt (Intra)		1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF Use a concentration of 1 µg/ml. This product gave a positive signal in wild-type HAP1 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
IP 1/150.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 129 kDa).
Flow Cyt (Intra) 1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Sequence similarities

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localization

Membrane.
Target information above from: UniProt accession P17301 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3673 Human
- Entrez Gene: 16398 Mouse
- Entrez Gene: 170921 Rat
- Omim: 192974 Human
- SwissProt: P17301 Human
- SwissProt: Q62469 Mouse
- Unigene: 482077 Human
- Unigene: 5007 Mouse
Alternative names
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
- CD49 antigen-like family member B antibody
- CD49b antibody
- CD49b antigen antibody
- Collagen receptor antibody
- DX5 antibody
- Glycoprotein Ia deficiency included antibody
- GP Ia antibody
- GP Ia deficiency, included antibody
- GPIa antibody
- HPA 5 included antibody
- HPA5 included antibody
- Human platelet alloantigen system 5 antibody
- Integrin alpha 2 antibody
- Integrin alpha II antibody
- Integrin alpha-2 antibody
- Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
- ITA2_HUMAN antibody
- ITGA2 antibody
- Platelet alloantigen Br(a), included antibody
- Platelet antigen Br antibody
- Platelet glycoprotein GPIa antibody
- Platelet glycoprotein Ia antibody
- Platelet glycoprotein Ia/IIa antibody
- Platelet membrane glycoprotein Ia antibody
- Platelet receptor for collagen, deficiency of, included antibody
- Very late activation protein 2 receptor alpha 2 subunit antibody
- VLA 2 alpha chain antibody
- VLA 2 antibody
- VLA 2 subunit alpha antibody
- VLA-2 subunit alpha antibody
- VLA2 antibody
- VLA2 receptor alpha 2 subunit antibody
- VLAA2 antibody
see all

Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: Integrin alpha 2 knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: A431 cell lysate (20 µg)
Lanes 4, 8 and 12: T47D cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target - ab181548 observed at 150 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal

ab181548 was shown to specifically react with Integrin alpha 2 when Integrin alpha 2 knockout samples were used. Wild-type and Integrin alpha 2 knockout samples were subjected to SDS-PAGE. ab181548 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

ab181548 staining Integrin α2 in wild-type HAP1 cells (top panel) and Integrin α2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab181548 at 1μg/ml concentration and ab7291 at 1ug/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2ug/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

All lanes : Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/20000 dilution

Lane 1 : A549 (Human lung carcinoma) whole cell lysates
Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysates
Lane 3 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human colon is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab181548 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

All lanes : Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/5000 dilution

Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Rat spleen tissue lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of Mouse kidney tubule is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane and weakly cytoplasmic staining on PC-3 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab181548 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling integrin alpha 2 with ab181549 at 1/160 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane staining on epithelial cells of Rat colon tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

All lanes : Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548) at 1/5000 dilution

Lane 1 : Human fetal brain whole cell lysates
Lane 2 : Human fetal heart whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 129 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer 5% NFDM/TBST.
The increased molecular mass observed is due to glycosylation.
Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Integrin alpha 2 was immunoprecipitated from 1mg of T-47D (Human ductal breast epithelial tumor cell line) whole cell extract with ab181548 at 1/150 dilution. Western blot was performed using ab181548 at 1/20,000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: T-47D whole cell extract Lane 2: PBS instead of T-47D whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab181548? Please let us know so that we can cite the reference in this datasheet.

ab181548 has been referenced in 16 publications.

Huang W et al. Exosomes with low miR-34c-3p expression promote invasion and migration of non-small cell lung cancer by upregulating integrin a2ß1. Signal Transduct Target Ther 5:39 (2020). PubMed: 32317629
Qin A et al. Ropivacaine inhibits proliferation, invasion, migration and promotes apoptosis of papillary thyroid cancer cells via regulating ITGA2 expression. Drug Dev Res N/A:N/A (2020). PubMed: 32314406
Vila-Navarro E et al. MiR-93 is related to poor prognosis in pancreatic cancer and promotes tumor progression by targeting microtubule dynamics. Oncogenesis 9:43 (2020). PubMed: 32366853
Sanyour HJ et al. Statin-mediated cholesterol depletion exerts coordinated effects on the alterations in rat vascular smooth muscle cell biomechanics and migration. J Physiol 598:1505-1522 (2020). PubMed: 32083311
Si X et al. circRNA Hipk3 Induces Cardiac Regeneration after Myocardial Infarction in Mice by Binding to Notch1 and miR-133a. Mol Ther Nucleic Acids 21:636-655 (2020). PubMed: 32736292

View all Publications for this product

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1-4 of 4 Abreviews

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Mouth floor approx)

Permeabilization

Specification

Mouth floor approx

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Acetone

Abcam user community

Verified customer

Submitted Aug 04 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

colon

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

Abcam user community

Verified customer

Submitted Mar 14 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

colon

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

Abcam user community

Verified customer

Submitted Mar 14 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Spleen)

Permeabilization

Specification

Spleen

Blocking step

BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Fixative

Formaldehyde

Mr. Simon Cleary

Verified customer

Submitted Jul 02 2015

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Immunogen

Positive control

General notes

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Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

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The Abpromise guarantee

Target

Function

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References (16)

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Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal