Product nameAnti-Integrin alpha 2 antibody [EPR17338] - C-terminal (HRP)
See all Integrin alpha 2 primary antibodies
DescriptionRabbit monoclonal [EPR17338] to Integrin alpha 2 - C-terminal (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Integrin alpha 2 aa 1150 to the C-terminus. The exact sequence is proprietary.
Database link: P17301
- WB: A549, T-47D, C6 and wild-type HAP1 whole cell lysates and human fetal brain tissue lysates. IHC-P: Normal human colon tissue sections.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab181548)
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (Alexa Fluor® 488) (ab208770)
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (Alexa Fluor® 647) (ab209766)
- Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (Alexa Fluor® 594) (ab210655)
- Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
- Anti-Integrin alpha 2 antibody [EPR17338] (Phycoerythrin) (ab225284)
Our Abpromise guarantee covers the use of ab209944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 129 kDa).|
FunctionIntegrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
IHC image of Integrin alpha 2 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209944, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (HRP) (ab209944) at 1/5000 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Lane 2 : T-47D (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : C6 (Rat glioma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Lane 5 : Wild-type HAP1 cell lysate at 20 µg
Lane 6 : Integrin alpha 2 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209944 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209944 has not yet been referenced specifically in any publications.