• Product name

    Anti-Integrin alpha 2 antibody [HAS-3]
    See all Integrin alpha 2 primary antibodies
  • Description

    Mouse monoclonal [HAS-3] to Integrin alpha 2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Whole human keratinocytes.

  • General notes

    The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.



Our Abpromise guarantee covers the use of ab10800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20435685
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • Function

    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • BR antibody
    • CD 49b antibody
    • CD49 antigen like family member B antibody
    • CD49 antigen-like family member B antibody
    • CD49b antibody
    • CD49b antigen antibody
    • Collagen receptor antibody
    • DX5 antibody
    • Glycoprotein Ia deficiency included antibody
    • GP Ia antibody
    • GP Ia deficiency, included antibody
    • GPIa antibody
    • HPA 5 included antibody
    • HPA5 included antibody
    • Human platelet alloantigen system 5 antibody
    • Integrin alpha 2 antibody
    • Integrin alpha II antibody
    • Integrin alpha-2 antibody
    • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
    • ITA2_HUMAN antibody
    • ITGA2 antibody
    • Platelet alloantigen Br(a), included antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet glycoprotein Ia/IIa antibody
    • Platelet membrane glycoprotein Ia antibody
    • Platelet receptor for collagen, deficiency of, included antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA 2 subunit alpha antibody
    • VLA-2 subunit alpha antibody
    • VLA2 antibody
    • VLA2 receptor alpha 2 subunit antibody
    • VLAA2 antibody
    see all


  • Immunohistochemical analysis of Human blood vessels, staining Integrin alpha 2 with ab10800.

    Tissue was fixed with formaldehyde and blocked with 3% goat serum for 30 minutes at 21°C; antigen retrieval was by an enzymatic method. Samples were incubated with primary antibody (1/10 in diluent) for 1 hour at 21°C. An undiluted streptavidin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of Human prostate cancer tissue, staining Integrin alpha 2 with ab10800. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFLuor®488-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.


This product has been referenced in:

  • van Leenders GJ  et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . Read more (PubMed: 22110593) »
  • Singh SR  et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . Read more (PubMed: 20435685) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Vessels)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase K
Blocking step
Goat-Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 06 2012



Expiration date: 01. March 2013

Value: €370.00

I am very pleased to hear you would like to use ab10800 in IHC and send us an image of your results.

This code will give you a €370.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview with the image for IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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I'm happy to help! Please let me know if you need anything further.

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Thanks for your enquiry.

My name is Jeremy Kasanov and I am filling in for Sybille while she is out of the office.

If you have submitted your review with the discount code in the 'Additional notes' section, you have doone all that is required. To claim your free antibody, just call our Customer Service department and tell them you would like to order your free antibody you are due do to your participation in the 100% testing discount promotion. The Customer Service rep will just confirm your review has been submitted with the correct code and will then award you the discount immediately.

I hope this is helpful. Please contact me again if you have any further questions.

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Thank you for submitting an Abreview for ab10800. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- loading more sample
- using more primary antibody (e.g. 1/500)

If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Read More
Western blot
Rat Cell lysate - whole cell (Uterine epithelial cells)
Loading amount
20 µg
Uterine epithelial cells
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% acrylamide)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 25 2012


Thank you for confirming that information.
I have generated the testing discount code as discussed:
Discount code: xxxxx Expiration date: 29th of September 2012
Once the Abreview detailing the results of the customers experiment has been submitted the code will become active.
The terms and conditions of this offer can be found from the following link:
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Thank you for getting back to me with that confirmation.
In answer to your question, yes that is how the testing discount works, regardless of if the results with the antibody are positive or negative and the Abreview is submitted with the details of the experiment carried out, then the customer would be entitled to a free primary antibody of their choice (or the price of the original antibody off any product in our catalogue). The only limitation is that the free product must be claimed within a four month period of purchasing the antibody to be tested.
This offer requires that a discount code is issued PRIOR TO the purchase of the antibody to be tested. Please make your customer aware of this. In this case I am willing to make an exception if the customer has already purchased the antibody. In order to generate the discount code could you please supply me with the email address and institution of the customer.
I hope this information has been of help. I look forward to receiving your reply.

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Thank you for contacting us.
The anti-integrin alpha 2 antibody (HAS-3), (ab10800) your customer is interested in has been raised against whole human keratinocytes. The human integrin alpha 2 protein shares 82% homology with the rat protein. It is therefore uncertain if the antibody would be able to detect the rat protein. I would however be able to offer a testing discount if your customer wanted to try the antibody with their rat samples.
In order to do this I will need the customers email address and institute.
As the antibody ab10800 has currently not been tested in Western blotting, I would suggest your customer might want to try ab55340 instead as this has been used in western blotting and the immunogen used shares 84% homology with the rat protein.
Please let me know how the customer would like to proceed.

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Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)  ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney  ab4287, ab4330, ab4347  ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus  ab4287, ab4330, ab4404  ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617  ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney  ab4372, ab4347  ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus  ab4287, ab4404  All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

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1-10 of 13 Abreviews or Q&A

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