Anti-Integrin alpha 2 antibody [HAS-3] (ab10800)


  • Product name
    Anti-Integrin alpha 2 antibody [HAS-3]
    See all Integrin alpha 2 primary antibodies
  • Description
    Mouse monoclonal [HAS-3] to Integrin alpha 2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IP, Flow Cyt, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Whole human keratinocytes.

  • General notes

    The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.



Our Abpromise guarantee covers the use of ab10800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20435685
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • BR antibody
    • CD 49b antibody
    • CD49 antigen like family member B antibody
    • CD49 antigen-like family member B antibody
    • CD49b antibody
    • CD49b antigen antibody
    • Collagen receptor antibody
    • DX5 antibody
    • Glycoprotein Ia deficiency included antibody
    • GP Ia antibody
    • GP Ia deficiency, included antibody
    • GPIa antibody
    • HPA 5 included antibody
    • HPA5 included antibody
    • Human platelet alloantigen system 5 antibody
    • Integrin alpha 2 antibody
    • Integrin alpha II antibody
    • Integrin alpha-2 antibody
    • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
    • ITA2_HUMAN antibody
    • ITGA2 antibody
    • Platelet alloantigen Br(a), included antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet glycoprotein Ia/IIa antibody
    • Platelet membrane glycoprotein Ia antibody
    • Platelet receptor for collagen, deficiency of, included antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA 2 subunit alpha antibody
    • VLA-2 subunit alpha antibody
    • VLA2 antibody
    • VLA2 receptor alpha 2 subunit antibody
    • VLAA2 antibody
    see all


  • Immunohistochemical analysis of Human blood vessels, staining Integrin alpha 2 with ab10800.

    Tissue was fixed with formaldehyde and blocked with 3% goat serum for 30 minutes at 21°C; antigen retrieval was by an enzymatic method. Samples were incubated with primary antibody (1/10 in diluent) for 1 hour at 21°C. An undiluted streptavidin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of Human prostate cancer tissue, staining Integrin alpha 2 with ab10800. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFLuor®488-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.


This product has been referenced in:
  • van Leenders GJ  et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . Read more (PubMed: 22110593) »
  • Singh SR  et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . Read more (PubMed: 20435685) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Vessels)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase K
Blocking step
Goat-Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 06 2012


Expiration date: 01. March 2013

Value: €370.00

I am very pleased to hear you would like to use ab10800 in IHC and send us an image of your results.

This code will give you a &eu...

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I'm happy to help! Please let me know if you need anything further.

Thanks for your enquiry.

My name is Jeremy Kasanov and I am filling in for Sybille while she is out of the office.

If you have submitted your review with the discount code in the 'Additional notes' section, you have doone all that i...

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Thank you for submitting an Abreview for ab10800. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species and application, we would like to follow ...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (Uterine epithelial cells)
Loading amount
20 µg
Uterine epithelial cells
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% acrylamide)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 25 2012

Thank you for confirming that information.
I have generated the testing discount code as discussed:
Discount code: xxxxx Expiration date: 29th of September 2012
Once the Abreview detailing the results of the customers experiment has been...

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Thank you for getting back to me with that confirmation.
In answer to your question, yes that is how the testing discount works, regardless of if the results with the antibody are positive or negative and the Abreview is submitted with the details...

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Thank you for contacting us.
The anti-integrin alpha 2 antibody (HAS-3), (ab10800) your customer is interested in has been raised against whole human keratinocytes. The human integrin alpha 2 protein shares 82% homology with the rat protein. It is...

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Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

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