Key features and details
- Mouse monoclonal [HAS-3] to Integrin alpha 2
- Suitable for: IP, Flow Cyt, IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-Integrin alpha 2 antibody [HAS-3]
See all Integrin alpha 2 primary antibodies
DescriptionMouse monoclonal [HAS-3] to Integrin alpha 2
Tested applicationsSuitable for: IP, Flow Cyt, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Whole human keratinocytes.
The antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Primary antibody notesThe antibody co-precipitates alpha 2 beta 1 integrin from keratinocytes and inhibits HGF induced branching morphogenesis and motility of human mammary epithelial HB2 cells in collagen.
Our Abpromise guarantee covers the use of ab10800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionIntegrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- BR antibody
- CD 49b antibody
- CD49 antigen like family member B antibody
Immunohistochemical analysis of Human blood vessels, staining Integrin alpha 2 with ab10800.
Tissue was fixed with formaldehyde and blocked with 3% goat serum for 30 minutes at 21°C; antigen retrieval was by an enzymatic method. Samples were incubated with primary antibody (1/10 in diluent) for 1 hour at 21°C. An undiluted streptavidin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.
Immunohistochemical analysis of Human prostate cancer tissue, staining Integrin alpha 2 with ab10800. Samples were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFLuor®488-conjugated anti-mouse IgG (1/100) was used as the secondary antibody.
ab10800 has been referenced in 5 publications.
- van Leenders GJ et al. Activation of c-MET Induces a Stem-Like Phenotype in Human Prostate Cancer. PLoS One 6:e26753 (2011). IHC-Fr ; Human . PubMed: 22110593
- Singh SR et al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF ; Human . PubMed: 20435685
- Alford D et al. Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels. J Cell Sci 111 Pt 4:521-32 (1998). PubMed: 9443900
- Sugiyama M et al. Comparison of integrin expression and terminal differentiation capacity in cell lines derived from oral squamous cell carcinomas. Carcinogenesis 14:2171-6 (1993). PubMed: 7693359
- Tenchini ML et al. Evidence against a major role for integrins in calcium-dependent intercellular adhesion of epidermal keratinocytes. Cell Adhes Commun 1:55-66 (1993). PubMed: 7521749