Anti-Integrin alpha 2+beta 1 antibody [16B4] (ab30483)


  • Product name
    Anti-Integrin alpha 2+beta 1 antibody [16B4]
    See all Integrin alpha 2+beta 1 primary antibodies
  • Description
    Mouse monoclonal [16B4] to Integrin alpha 2+beta 1
  • Host species
  • Specificity
    We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ICC/IF, IP, Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.

  • Positive control
    • ICC-IF: A431 cells
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab30483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 10 µg/ml.
IP Use a concentration of 25 - 50 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ELISA Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 30 - 40 µg/ml.


  • Cellular localization
    Cell Membrane; single-pass type I membrane protein
  • Database links
  • Alternative names
    • CD29 antibody
    • CD49B antibody
    • Collagen receptor antibody
    • GPIa antibody
    • GPIIA antibody
    • Integrin alpha 2 antibody
    • Integrin beta 1 antibody
    • ITGA2 antibody
    • ITGB1 antibody
    • Platelet antigen Br antibody
    • Platelet glycoprotein GPIa antibody
    • Platelet glycoprotein Ia antibody
    • Platelet membrane glycoprotein Ia antibody
    • Very late activation protein 2 receptor alpha 2 subunit antibody
    • VLA 2 alpha chain antibody
    • VLA 2 antibody
    • VLA4 antibody
    • VLAA2 antibody
    see all


  • Overlay histogram showing HT1080 cells stained with ab30483 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab30483, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HT1080 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab30483 stained in A431cells. The cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1 hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with ab30483 at 5 µg/ml and ab6046 (Rabbit polyclonal to beta tubulin) at 1 ug/ml overnight at +4°C. The secondary antibodies were ab150117 (colored green) used at 1 ug/ml and ab150087 (pseudo-colored red) used at 2 µg/ml for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature.

  • ab30483 staining human breast cancer cells by ICC/IF.  Cells were PFA fixed and blocked with 1% serum for 16 hours at 20°C prior to incubating with ab30483 (at 30µg/ml) for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/200, was used as the secondary.

    See Abreview


This product has been referenced in:
  • Mrazkova B  et al. Induction, regulation and roles of neural adhesion molecule L1CAM in cellular senescence. Aging (Albany NY) 10:434-462 (2018). WB . Read more (PubMed: 29615539) »
  • Chung CH  et al. Aggretin Venom Polypeptide as a Novel Anti-angiogenesis Agent by Targeting Integrin alpha2beta1. Sci Rep 7:43612 (2017). Read more (PubMed: 28252668) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

Based on what you have already tested, I am not sure there are many more steps to be optimized. One consideration would be to extend the primary antibody incubation to overnight at 4oC. Longer incubation with l...

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Prostate Cancer cells (PC3))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Anticancer drug
Prostate Cancer cells (PC3)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 09 2016

Thank you once again for your telephone enquiry.

I am sorry to confirm that this antibody has been tested externally in ELISA and regrettably we have no further details to provide on the protocol on this occasion.

We aim to provid...

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Thank you for your enquiry.

I am sorry I am not able to confirm much further detail regarding the immunogen for ab30483 Anti-Integrin alpha 2+beta 1 antibodyas this particular product has been sourced externally.

However, I can co...

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Thank you for getting back to me with an update of your progress.

I have gone back and had a look at the previous results of your colleague. There definitely seems to be some staining of fibroblasts (in the boiled sample). As you say, it may ...

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Thank you for providing that information. It has helped greatly in understanding what you have been observing.

To answer your question about what staining you can expect with anti-Integrin alpha 2+beta 1 antibody (ab30483) in human skin...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (Breast Cancer Cells)
Breast Cancer Cells
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Dr. Sharon Sneddon

Verified customer

Submitted Jul 10 2008


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