Key features and details
- Goat polyclonal to Integrin alpha 3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Integrin alpha 3 antibody
See all Integrin alpha 3 primary antibodies
DescriptionGoat polyclonal to Integrin alpha 3
SpecificityThis antibody is expected to recognize reported isoform a (NP_002195.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Chinese hamster
- WB: Human heart lysate; mouse heart lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 117 kDa).
1 hour primary incubation is recommended for this product.
FunctionIntegrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
Tissue specificityIsoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
modificationsIsoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
- Information by UniProt
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
Lane 1 : Anti-Integrin alpha 3 antibody (ab223661) at 1 µg/ml
Lane 2 : Anti-Integrin alpha 3 antibody (ab223661) at 1 mg/ml
Lane 1 : Human heart lysate
Lane 2 : Mouse heart lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 117 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab223661 has not yet been referenced specifically in any publications.