Overview

  • Product name

    Anti-Integrin alpha 3 antibody
    See all Integrin alpha 3 primary antibodies
  • Description

    Goat polyclonal to Integrin alpha 3
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize reported isoform a (NP_002195.1).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Integrin alpha 3 aa 1038-1050 (C terminal) (Cysteine residue).
    Sequence:

    C-KSQPSETERLTDD


    Database link: P26006

  • Positive control

    • WB: Human heart lysate; mouse heart lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 117 kDa).

Target

  • Function

    Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
  • Tissue specificity

    Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications

    Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA407068 antibody
    • Antigen identified by monoclonal antibody J143 antibody
    • CD49 antigen like family member C antibody
    • CD49 antigen-like family member C antibody
    • CD49c antibody
    • CD49c antigen antibody
    • FLJ34631 antibody
    • FLJ34704 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • Galactoprotein B3 antibody
    • GAP B3 antibody
    • GAPB3 antibody
    • Integrin alpha 3 antibody
    • Integrin alpha-3 light chain antibody
    • Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) antibody
    • ITA3_HUMAN antibody
    • ITGA 3 antibody
    • Itga3 antibody
    • MSK18 antibody
    • VCA 2 antibody
    • VCA2 antibody
    • Very late activation protein 3 receptor alpha 3 subunit antibody
    • VL3A antibody
    • VLA 3 alpha chain antibody
    • VLA 3 subunit alpha antibody
    • VLA-3 subunit alpha antibody
    • VLA3a antibody
    see all

Images

  • Lane 1 : Anti-Integrin alpha 3 antibody (ab223661) at 1 µg/ml
    Lane 2 : Anti-Integrin alpha 3 antibody (ab223661) at 1 mg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Mouse heart lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 117 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour

References

ab223661 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223661.
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