Anti-Integrin alpha 3 antibody [ASC-1] (ab228425)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin alpha 3 antibody [ASC-1]
See all Integrin alpha 3 primary antibodies -
Description
Mouse monoclonal [ASC-1] to Integrin alpha 3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Integrin alpha 3. (Human SSC-9 cell line (squamous cell carcinoma)).
Database link: P26006 -
Positive control
- Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity: > 95% (by SDS-PAGE). Purified from TCS. -
Clonality
Monoclonal -
Clone number
ASC-1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab228425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. -
Tissue specificity
Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3675 Human
- Omim: 605025 Human
- SwissProt: P26006 Human
- Unigene: 265829 Human
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Alternative names
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
see all
Images
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![Flow Cytometry - Anti-Integrin alpha 3 antibody [ASC-1] (ab228425)](https://www.abcam.com/ps/products/228/ab228425/Images/ab228425-293213-anti-integrin-alpha-3-antibody-asc-1-flow-cytometry.jpg)
Flow Cytometry - Anti-Integrin alpha 3 antibody [ASC-1] (ab228425)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Integrin alpha 3 with ab228425, GAM-APC (red) compared to a control (grey).
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![Flow Cytometry - Anti-Integrin alpha 3 antibody [ASC-1] (ab228425)](https://www.abcam.com/ps/products/228/ab228425/Images/ab228425-293214-anti-integrin-alpha-3-antibody-asc-1-flow-cytometry.jpg)
Flow Cytometry - Anti-Integrin alpha 3 antibody [ASC-1] (ab228425)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Integrin alpha 3 with ab228425, PE (red) compared to a control (blue).
Datasheets and documents
References
ab228425 has not yet been referenced specifically in any publications.
