Anti-Integrin alpha 3 antibody [F35 177-1] (ab20140)
Key features and details
- Mouse monoclonal [F35 177-1] to Integrin alpha 3
- Suitable for: IHC-Fr, IHC-FoFr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Integrin alpha 3 antibody [F35 177-1]
See all Integrin alpha 3 primary antibodies -
Description
Mouse monoclonal [F35 177-1] to Integrin alpha 3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-FoFrmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Integrin alpha 3. MTSV1-7 HYE - human breast epithelial cells
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General notes
Fusion Partner: NS2The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
F35 177-1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (1) |
Use at an assay dependent concentration. Fix with acetone.
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IHC-FoFr |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. Fix with acetone. |
IHC-FoFr
Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. -
Tissue specificity
Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. Isoform alpha-3A is phosphorylated on Tyr-1051. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3675 Human
- Omim: 605025 Human
- SwissProt: P26006 Human
- Unigene: 265829 Human
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Alternative names
- AA407068 antibody
- Antigen identified by monoclonal antibody J143 antibody
- CD49 antigen like family member C antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab20140 has been referenced in 1 publication.
- Lustosa SA et al. Expression profiling using a cDNA array and immunohistochemistry for the extracellular matrix genes FN-1, ITGA-3, ITGB-5, MMP-2, and MMP-9 in colorectal carcinoma progression and dissemination. ScientificWorldJournal 2014:102541 (2014). IHC ; Human . PubMed: 24737953