• Product name
    Anti-Integrin alpha 4 antibody [44H6]
    See all Integrin alpha 4 primary antibodies
  • Description
    Mouse monoclonal [44H6] to Integrin alpha 4
  • Host species
  • Specificity
    Recognises the integrin alpha 4 subunit, which associates with either Integrin beta 1 (to form VLA 4) or beta 7. The alpha 4 integrin is a 150 kDa molecule, which may be cleaved to 80 kDa and 70 kDa fragments.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HOON pre-B leukaemia cell line.

  • Positive control
    • Flow Cyt: Human whole peripheral blood cells. IHC-Fr: Human tonsil tissue. ICC/IF: Wild-type HAP-1 cells.



Our Abpromise guarantee covers the use of ab220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/40.

Use 10 µl of the working dilution to label 100 µl of human whole peripheral blood.




ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-Fr 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.
IP 1/20 - 1/25.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Domain
      The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
    • Post-translational
      Phosphorylation on Ser-1027 inhibits PXN binding.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • 269C wild type antibody
      • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
      • CD49 antigen like family member D antibody
      • CD49 antigen-like family member D antibody
      • CD49d antibody
      • IA4 antibody
      • Integrin alpha 4 antibody
      • Integrin alpha 4 subunit antibody
      • Integrin alpha IV antibody
      • Integrin alpha-4 antibody
      • Integrin alpha-IV antibody
      • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
      • ITA4_HUMAN antibody
      • ITGA4 antibody
      • MGC90518 antibody
      • OTTHUMP00000205320 antibody
      • Very late activation protein 4 receptor, alpha 4 subunit antibody
      • VLA 4 subunit alpha antibody
      • VLA-4 subunit alpha antibody
      • VLA4 antibody
      see all


    • ab220 staining Integrin alpha 4 in wild-type HAP1 cells (top panel) and Integrin alpha 4 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab220 at 5μg/ml concentration and ab220272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    • Staining of human peripheral blood lymphocytes with ab220.


    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - other (blood Natural Killer cells)
    Loading amount
    10 µg
    blood Natural Killer cells
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 17 2008


    Thank you for your enquiry. This product recognises the extracellular portion of integrin alpha 4. However, since epitope mapping was never done, there is no information on the exact epitope sequence.

    Read More


    Thank you for your enquiry regarding ab220. We have not mapped the epitope region recognized by this antibody therefore we do not know which region is recognized by the antibody. Sorry I couldn't help you more on this occasion,

    Read More


    Thank you for your email. We have had feedback from a customer who used this antibody at a 1:200 dilution for Western blotting. I'm really not sure why this antibody is not working for you, and can offer (if you have purchased the antibody in the past 90 days) a replacement vial to try or a refund or credit note. Please let me know how you would like to proceed.

    Read More


    Thank you for your email. I noticed in your previous email that you mentioned for a positive control, "tried 2 different previously verified positive controls." Can you please elaborate on that? For your other samples, the soluble integrin alpha 4 secreted into cell supernatant, I wonder if there's enough present there to be detected by this antibody. Thanks again, and I look forward to hearing from you.

    Read More


    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with this antibody. Did you determine how much protein was loaded onto the gel? Also, do you know if the protein transferred properly to the membrane? What was the batch number (located on the vial) or the Abcam order number or purchase order number that was used when you purchased ab220? Thank you, and I look forward to hearing from you.

    Read More


    Sign up