Anti-Integrin alpha 4/CD49D antibody (ab202969)
Key features and details
- Rabbit polyclonal to Integrin alpha 4/CD49D
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Integrin alpha 4/CD49D antibody
See all Integrin alpha 4/CD49D primary antibodies -
Description
Rabbit polyclonal to Integrin alpha 4/CD49D -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Integrin alpha 4/CD49D aa 584-609 conjugated to keyhole limpet haemocyanin.
Sequence:GPHVISKRSTEEFPPLQPILQQKKEK
Database link: P13612 -
General notes
This product was previously labelled as Integrin alpha 4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab202969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P | 1/100 - 1/500. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
-
Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
Post-translational
modificationsPhosphorylation on Ser-1027 inhibits PXN binding. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3676 Human
- Entrez Gene: 16401 Mouse
- Entrez Gene: 311144 Rat
- Omim: 192975 Human
- SwissProt: P13612 Human
- SwissProt: Q00651 Mouse
- Unigene: 440955 Human
- Unigene: 31903 Mouse
-
Alternative names
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
Images
-
Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (ab202969)Flow Cytometry analysis of Human U937 cells labeling Integrin alpha 4/CD49D with ab202969 at 0.2 µg for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes. Blue - control cells, light blue - secondary only, orange - isotype control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (ab202969)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung labeling CD49d with ab202969 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Protocols
References (2)
ab202969 has been referenced in 2 publications.
- Nakane Y et al. Hyperglycemia contributes to the development of Leydig cell hyperplasia in male Spontaneously Diabetic Torii rats. J Toxicol Pathol 33:121-129 (2020). PubMed: 32425345
- Yang TX et al. The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats. Cell Physiol Biochem 46:36-45 (2018). PubMed: 29566360
