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  1. Link

    integrin-alpha-4cd49d-antibody-ab202969.pdf

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Immunology Cell Type Markers CD Non-lineage
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Anti-Integrin alpha 4/CD49D antibody (ab202969)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (ab202969)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (ab202969)

Key features and details

  • Rabbit polyclonal to Integrin alpha 4/CD49D
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Chimeric (ab275979)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D antibody
    See all Integrin alpha 4/CD49D primary antibodies
  • Description

    Rabbit polyclonal to Integrin alpha 4/CD49D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Integrin alpha 4/CD49D aa 550-650 conjugated to keyhole limpet haemocyanin.
    Database link: P13612

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Integrin alpha 4/CD49D protein (ab158773)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab202969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P
1/100 - 1/500.

Use at 1/50 - 1/200 with fluorescent detection methods.

Notes
Flow Cyt
1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P
1/100 - 1/500.

Use at 1/50 - 1/200 with fluorescent detection methods.

Target

  • Function

    Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Domain

    The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • Post-translational
    modifications

    Phosphorylation on Ser-1027 inhibits PXN binding.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P13612 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3676 Human
    • Entrez Gene: 16401 Mouse
    • Omim: 192975 Human
    • SwissProt: P13612 Human
    • SwissProt: Q00651 Mouse
    • Unigene: 440955 Human
    • Unigene: 31903 Mouse
    • Alternative names

      • 269C wild type antibody
      • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
      • CD49 antigen like family member D antibody
      • CD49 antigen-like family member D antibody
      • CD49d antibody
      • IA4 antibody
      • Integrin alpha 4 antibody
      • Integrin alpha 4 subunit antibody
      • Integrin alpha IV antibody
      • Integrin alpha-4 antibody
      • Integrin alpha-IV antibody
      • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
      • ITA4_HUMAN antibody
      • ITGA4 antibody
      • MGC90518 antibody
      • OTTHUMP00000205320 antibody
      • Very late activation protein 4 receptor, alpha 4 subunit antibody
      • VLA 4 subunit alpha antibody
      • VLA-4 subunit alpha antibody
      • VLA4 antibody
      see all

    Images

    • Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (ab202969)
      Flow Cytometry - Anti-Integrin alpha 4/CD49D antibody (ab202969)

      Flow Cytometry analysis of Human U937 cells labeling Integrin alpha 4/CD49D with ab202969 at 0.2 µg for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes. Blue - control cells, light blue - secondary only, orange - isotype control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (ab202969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 4/CD49D antibody (ab202969)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung labeling CD49d with ab202969 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab202969? Please let us know so that we can cite the reference in this datasheet.

    ab202969 has been referenced in 7 publications.

    • Wei Q  et al. TNFa secreted by glioma associated macrophages promotes endothelial activation and resistance against anti-angiogenic therapy. Acta Neuropathol Commun 9:67 (2021). PubMed: 33853689
    • Su VY  et al. Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury. Int J Mol Sci 22:N/A (2021). PubMed: 34074039
    • Ma C  et al. Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche. Sci Adv 6:N/A (2020). PubMed: 33127669
    • Baiula M  et al. Leukocyte Integrin Antagonists as a Novel Option to Treat Dry Age-Related Macular Degeneration. Front Pharmacol 11:617836 (2020). PubMed: 33584300
    • Nakane Y  et al. Hyperglycemia contributes to the development of Leydig cell hyperplasia in male Spontaneously Diabetic Torii rats. J Toxicol Pathol 33:121-129 (2020). PubMed: 32425345
    • Magdaleno C  et al. HIFa independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl2. Sci Rep 10:18560 (2020). PubMed: 33122751
    • Yang TX  et al. The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats. Cell Physiol Biochem 46:36-45 (2018). PubMed: 29566360

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Integrin alpha 4/CD49D antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 24 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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