Overview

  • Product name

    Anti-Integrin alpha 4/CD49D antibody
    See all Integrin alpha 4/CD49D primary antibodies
  • Description

    Rabbit polyclonal to Integrin alpha 4/CD49D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Integrin alpha 4/CD49D aa 982-1032. The exact sequence is proprietary.
    Database link: P13612

  • Positive control

    • WB: Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as Integrin alpha 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 115 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Domain

      The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
    • Post-translational
      modifications

      Phosphorylation on Ser-1027 inhibits PXN binding.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 269C wild type antibody
      • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
      • CD49 antigen like family member D antibody
      • CD49 antigen-like family member D antibody
      • CD49d antibody
      • IA4 antibody
      • Integrin alpha 4 antibody
      • Integrin alpha 4 subunit antibody
      • Integrin alpha IV antibody
      • Integrin alpha-4 antibody
      • Integrin alpha-IV antibody
      • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
      • ITA4_HUMAN antibody
      • ITGA4 antibody
      • MGC90518 antibody
      • OTTHUMP00000205320 antibody
      • Very late activation protein 4 receptor, alpha 4 subunit antibody
      • VLA 4 subunit alpha antibody
      • VLA-4 subunit alpha antibody
      • VLA4 antibody
      see all

    Images

    • All lanes : Anti-Integrin alpha 4/CD49D antibody (ab226362) at 0.1 µg/ml

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
      Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
      Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 115 kDa


      Exposure time: 30 seconds


      Cell lysates were prepared using NETN lysis buffer.

    References

    ab226362 has not yet been referenced specifically in any publications.

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