Key features and details
- Rabbit polyclonal to Integrin alpha 4/CD49D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Integrin alpha 4/CD49D antibody
See all Integrin alpha 4/CD49D primary antibodies
DescriptionRabbit polyclonal to Integrin alpha 4/CD49D
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Integrin alpha 4/CD49D aa 982-1032. The exact sequence is proprietary.
Database link: P13612
- WB: Jurkat whole cell lysate.
This product was previously labelled as Integrin alpha 4
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226362 was affinity purified using an epitope specific to Integrin alpha 4/CD49D immobilized on solid support.
Our Abpromise guarantee covers the use of ab226362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 115 kDa.|
FunctionIntegrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
DomainThe SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
modificationsPhosphorylation on Ser-1027 inhibits PXN binding.
- Information by UniProt
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
All lanes : Anti-Integrin alpha 4/CD49D antibody (ab226362) at 0.1 µg/ml
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 115 kDa
Exposure time: 30 seconds
Cell lysates were prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226362 has not yet been referenced specifically in any publications.