Recombinant Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-253282-ab81280KOWB.jpg "Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Integrin alpha 4/CD49D antibody [EPR1355Y]
See all Integrin alpha 4/CD49D primary antibodies -
Description
Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Integrin alpha 4/CD49D aa 1000-1100 (C terminal). The exact sequence is proprietary.
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Positive control
- Jurkat whole cell lysate (ab7899)
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as Integrin alpha 4.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1355Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 150 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 150 kDa. |
Target
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Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
Post-translational
modificationsPhosphorylation on Ser-1027 inhibits PXN binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3676 Human
- Entrez Gene: 16401 Mouse
- Omim: 192975 Human
- SwissProt: P13612 Human
- SwissProt: Q00651 Mouse
- Unigene: 440955 Human
- Unigene: 31903 Mouse
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Alternative names
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
Images
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Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
Lane 1: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)
Lane 2: Wild-type HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-282047-ab81280.jpg)
Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)All lanes : Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution
All lanes : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 150 kDaLanes 1 - 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/Integrin-alpha-4-Primary-antibodies-ab81280-1.jpg)
Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa -
![Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-5-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
References (9)
ab81280 has been referenced in 9 publications.
- Yan J et al. Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K-Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. J Cell Mol Med 24:814-829 (2020). PubMed: 31680442
- Ohkuro M et al. Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. Nat Commun 9:1982 (2018). PubMed: 29773794
- Mishra P et al. ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming. J Clin Invest 128:323-340 (2018). PubMed: 29202474
- Zhang YL et al. SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways. Cancer Res 78:2305-2317 (2018). PubMed: 29440144
- van de Weijer ML et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). PubMed: 28743740
- de la Rica L et al. NF-?B-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol 16:2 (2015). WB . PubMed: 25601191
- Bing T et al. Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers. Mol Cell Proteomics 14:2692-700 (2015). WB ; Human . PubMed: 26199357
- Buggins AG et al. Evidence for a macromolecular complex in poor prognosis CLL that contains CD38, CD49d, CD44 and MMP-9. Br J Haematol 154:216-22 (2011). IF, WB ; Human . PubMed: 21569005
- Conant CG et al. Well plate-coupled microfluidic devices designed for facile image-based cell adhesion and transmigration assays. J Biomol Screen 15:102-6 (2010). PubMed: 19965806
