Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)

Overview

  • Product name

    Anti-Integrin alpha 4/CD49D antibody [EPR1355Y]
    See all Integrin alpha 4/CD49D primary antibodies
  • Description

    Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Integrin alpha 4/CD49D aa 1000-1100 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     This product was previously labelled as Integrin alpha 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 150 kDa.

Target

  • Function

    Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Domain

    The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
  • Post-translational
    modifications

    Phosphorylation on Ser-1027 inhibits PXN binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 269C wild type antibody
    • Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
    • CD49 antigen like family member D antibody
    • CD49 antigen-like family member D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin alpha-4 antibody
    • Integrin alpha-IV antibody
    • Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor) antibody
    • ITA4_HUMAN antibody
    • ITGA4 antibody
    • MGC90518 antibody
    • OTTHUMP00000205320 antibody
    • Very late activation protein 4 receptor, alpha 4 subunit antibody
    • VLA 4 subunit alpha antibody
    • VLA-4 subunit alpha antibody
    • VLA4 antibody
    see all

Images

  • Lane 1: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)

    Lane 2: Wild-type HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.


    ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • All lanes : Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution

    All lanes : Jurkat whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 150 kDa
    Observed band size: 150 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Secondary
    HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 150 kDa
    Observed band size: 150 kDa

References

This product has been referenced in:

  • Ohkuro M  et al. Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. Nat Commun 9:1982 (2018). Read more (PubMed: 29773794) »
  • Mishra P  et al. ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming. J Clin Invest 128:323-340 (2018). Read more (PubMed: 29202474) »
See all 8 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

The immunogen for ab81280 corresponds to an intracellular region of the protein:

http://www.uniprot.org/blast/?about=P13612[1002-1032]

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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