Recombinant Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Rabbit IgG (Chimeric) (ab275979)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Natalizumab] to Integrin alpha 4/CD49D - Chimeric
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Integrin alpha 4/CD49D antibody [Natalizumab] - Rabbit IgG (Chimeric)
See all Integrin alpha 4/CD49D primary antibodies -
Description
Rabbit monoclonal [Natalizumab] to Integrin alpha 4/CD49D - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Integrin alpha 4/CD49D. The RAMOS cell line, a non-EBV releasing cell line, injected into mice.
Database link: P13612 -
Positive control
- Flow cyt: Human lymphocytes.
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General notes
This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Natalizumab -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 1 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 1 µg/ml. |
Target
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Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
Post-translational
modificationsPhosphorylation on Ser-1027 inhibits PXN binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3676 Human
- Omim: 192975 Human
- SwissProt: P13612 Human
- Unigene: 440955 Human
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Alternative names
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
Images
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Flow cytometry of human lymphocytes labeling Integrin alpha 4/CD49D with ab275979 (panel B) compared with an isotype control (panel A) at 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)) and cells analysed on a FACSCanto flow-cytometer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab275979 has not yet been referenced specifically in any publications.