Overview

  • Product name
    Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)]
    See all Integrin alpha 5 primary antibodies
  • Description
    Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5
  • Host species
    Rat
  • Specificity
    ab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
  • Tested applications
    Suitable for: Functional Studies, ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)

  • General notes


    Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab25251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

T-cell co-stimulation, In vitro blocking of adhesion.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use up to 1ug/106 cells.

 

 

 

 

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Images

  • ab25251 staining Integrin alpha 5 in Mouse Embryonic Fibroblast (MEF) cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in Triton X-100. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 546-conjugated goat anti-rat polyclonal, diluted 1/200.

    See Abreview

References

This product has been referenced in:
  • Wang X  et al. Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of a5ß1 integrin expression. Int J Mol Med 38:737-46 (2016). Human . Read more (PubMed: 27460641) »
  • Rich S  et al. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol 157:2916-23 (1996). Read more (PubMed: 8816397) »
See all 6 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

I can confirm that the soluble Anti-Integrin alpha 5 antibody (clone 5H10-27 (MFR5)) was used to inhibit VLA5-mediated functions in vitro.

Since applications vary, we recommend to determine the optimum working dilution of the product that is appropriate for a specific need. More information about the use of the ab25251 to block the function of alpha 5 beta 1 fibronectin receptor can be found in the following literature. I hope that might be helpful to you to find a suitable starting dilution.

http://www.ncbi.nlm.nih.gov/pubmed?term=Halvorson%20%201995.%20J.%20Immunol.%20155%3A4567

http://www.ncbi.nlm.nih.gov/pubmed?term=Rich%20%2C%20van%20Nood%20and%20H.-M%20Lee.%201996.%20J.%20Immunol.%20157%3A2916.

http://www.ncbi.nlm.nih.gov/pubmed?term=Uhlenkott%2C%20C.E.%2C%20J.C.%20Huijzer%2C%20D.J.%20Cardeiro%2C%20C.A.%20Elstad%2C%20and%20G.G.%20Meadows.%201996.%20Clin.%20Exp.%20Metastasis%2014%3A125.

http://www.ncbi.nlm.nih.gov/pubmed?term=Schultz%2C%20J.F.%2C%20and%20D.R.%20Armant.%201995.%20J.%20Biol%20Chem.%20270%3A11522.



Regrettably I can not provide information about the epitope and were the product binds. I can confirm that the product is sourced external and no epitope mapping was performed, so the location of binding is unknown. It has been suggested that this clone reacts with the a5 chain of the integrin a5b1 fibronectin receptor on a variety of mouse cells and cell lines.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela cell line ( cervical cancer))
Specification
Hela cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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