Anti-Integrin alpha 5 antibody (ab226816)
Key features and details
- Rabbit polyclonal to Integrin alpha 5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Integrin alpha 5 antibody
See all Integrin alpha 5 primary antibodies -
Description
Rabbit polyclonal to Integrin alpha 5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Integrin alpha 5 (C terminal). The exact sequence is proprietary.
Database link: P08648 -
Positive control
- WB: MDA-MB-231 whole cell and membrane extract lysate; mouse and rat liver tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 115 kDa. |
Target
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Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3678 Human
- Entrez Gene: 16402 Mouse
- Entrez Gene: 315346 Rat
- Omim: 135620 Human
- SwissProt: P08648 Human
- SwissProt: P11688 Mouse
- Unigene: 505654 Human
- Unigene: 16234 Mouse
see all -
Alternative names
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Images
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Western blot - Anti-Integrin alpha 5 antibody (ab226816)All lanes : Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 membrane extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa -
Western blot - Anti-Integrin alpha 5 antibody (ab226816)Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Mouse liver tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa -
Western blot - Anti-Integrin alpha 5 antibody (ab226816)Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Rat liver tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab226816 has been referenced in 1 publication.
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
