Anti-Integrin alpha 5 antibody (ab226816)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin alpha 5 antibody
See all Integrin alpha 5 primary antibodies -
Description
Rabbit polyclonal to Integrin alpha 5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Integrin alpha 5 (C terminal). The exact sequence is proprietary.
Database link: P08648 -
Positive control
- WB: MDA-MB-231 whole cell and membrane extract lysate; mouse and rat liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 115 kDa. |
Target
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Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3678 Human
- Entrez Gene: 16402 Mouse
- Entrez Gene: 315346 Rat
- Omim: 135620 Human
- SwissProt: P08648 Human
- SwissProt: P11688 Mouse
- Unigene: 505654 Human
- Unigene: 16234 Mouse
see all -
Alternative names
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Images
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Western blot - Anti-Integrin alpha 5 antibody (ab226816)All lanes : Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 membrane extract lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa -
Western blot - Anti-Integrin alpha 5 antibody (ab226816)Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Mouse liver tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa -
Western blot - Anti-Integrin alpha 5 antibody (ab226816)Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Rat liver tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 115 kDa
References
ab226816 has not yet been referenced specifically in any publications.
