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    integrin-alpha-5-antibody-ab226816.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Anti-Integrin alpha 5 antibody (ab226816)

  • Datasheet
  • SDS
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Western blot - Anti-Integrin alpha 5 antibody (ab226816)
  • Western blot - Anti-Integrin alpha 5 antibody (ab226816)
  • Western blot - Anti-Integrin alpha 5 antibody (ab226816)

Key features and details

  • Rabbit polyclonal to Integrin alpha 5
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Human Integrin alpha 5 protein (ab151845)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Integrin alpha 5 antibody
    See all Integrin alpha 5 primary antibodies
  • Description

    Rabbit polyclonal to Integrin alpha 5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Integrin alpha 5 (C terminal). The exact sequence is proprietary.
    Database link: P08648

  • Positive control

    • WB: MDA-MB-231 whole cell and membrane extract lysate; mouse and rat liver tissue lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
  • Recombinant Protein

    • Recombinant Human Integrin alpha 5 protein (ab151845)
  • Related Products

    • Recombinant Human Integrin alpha V protein (Tagged) (ab114240)

Applications

Our Abpromise guarantee covers the use of ab226816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 115 kDa.

Target

  • Function

    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P08648 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3678 Human
    • Entrez Gene: 16402 Mouse
    • Entrez Gene: 315346 Rat
    • Omim: 135620 Human
    • SwissProt: P08648 Human
    • SwissProt: P11688 Mouse
    • Unigene: 505654 Human
    • Unigene: 16234 Mouse
    • Unigene: 100796 Rat
    see all
  • Alternative names

    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Images

  • Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    All lanes : Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : MDA-MB-231 membrane extract lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa

  • Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Mouse liver tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa

  • Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    Western blot - Anti-Integrin alpha 5 antibody (ab226816)
    Anti-Integrin alpha 5 antibody (ab226816) at 1/5000 dilution + Rat liver tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 115 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab226816? Please let us know so that we can cite the reference in this datasheet.

    ab226816 has been referenced in 1 publication.

    • Fu Y  et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750

    Customer reviews and Q&As

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