Anti-Integrin alpha 5 antibody [SAM-1] (Biotin) (ab95714)


  • Product name
    Anti-Integrin alpha 5 antibody [SAM-1] (Biotin)
    See all Integrin alpha 5 primary antibodies
  • Description
    Mouse monoclonal [SAM-1] to Integrin alpha 5 (Biotin)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    U937 cells

  • Positive control
    • Human PBMCs



Our Abpromise guarantee covers the use of ab95714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25-0.5µg for 105-8 cells.

Staining the cell sample in a final volume of 100 µl is recommended.




ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all


  • Flow cytometry staining of normal Human peripheral blood cells with Anti-Human CD3 FITC and 0.25 µg of Mouse IgG1 ? Isotype Control Biotin (left) or 0.25 µg of ab95714 (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.


This product has been referenced in:
  • Grassi F  et al. Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol 64:484-93 (1998). Read more (PubMed: 9766629) »
  • Xiao J  et al. Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases. J Biol Chem 271:7659-64 (1996). Flow Cyt . Read more (PubMed: 8631803) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

I apologise for the delay in getting back to you. I have been revewing and checking the immunogen sequences of our Integrin alpha 5 antibody. Regrettably, so far I am sorry I have not found one that would match yo...

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Thank you for the details. For intergrin beta 1, the antibody ab23825 might recognize the CHO protein but it has not been tested with this speices. It does apparently recoagnize an extracellular epitope, as the clone is reactive with mouse CD29 can ...

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Thank you for contacting us. These might work but it will depend on what kind of integrin the cells are expressing. Are these cells transfected with integrin DNA, or is the integrin endogenous CHO integrin.? If transfected, do you know the species and ...

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