Overview

  • Product name

    Anti-Integrin alpha 6 antibody
    See all Integrin alpha 6 primary antibodies
  • Description

    Rabbit polyclonal to Integrin alpha 6
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, a wide range of other species
  • Immunogen

    Synthetic peptide, raised against an 18 amino acid sequence from N terminal of Human Integrin alpha 6.

  • Positive control

    • Human breast tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab133386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • Involvement in disease

    Epidermolysis bullosa letalis, with pyloric atresia
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • Cellular localization

    Cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded human stomach labelling Integrin alpha 6 with ab133386 at 1 µg/ml. Followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human breast tissue labelling Integrin alpha 6 with ab133386 at 1 µg/ml. Followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab133386 has not yet been referenced specifically in any publications.

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