Recombinant
RabMAb

Recombinant Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18124] to Integrin alpha 6
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin alpha 6 antibody [EPR18124]
    See all Integrin alpha 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR18124] to Integrin alpha 6

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Integrin alpha 6 aa 1-100. The exact sequence is proprietary.
    Database link: P23229

  • Positive control

    • WB: SW480, A549, F6 whole cell lysates; Mouse skin lysate and Rat skin lysate, Human fetal kidney tissue lysate, Mouse kidney and spleen tissue lysate, Rat spleen tissue lysate. IHC-P: Human breast tissue, Human endometrial cancer tissue, Mouse stomach tissue, Rat stomach tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab181551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).

  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).

  • Involvement in disease

    Epidermolysis bullosa letalis, with pyloric atresia

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • Post-translational
    modifications

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.

  • Cellular localization

    Cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alpha6p antibody
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • Processed integrin alpha-6 antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.  

      Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Human endometrial cancer  tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

      Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma) whole cell lysate
      Lane 3 : Mouse skin lysate
      Lane 4 : Rat skin lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST.

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 10 seconds


      Blocking and diluting buffer was 5% NFDM /TBST

    • Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Western blot - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

      Lane 1 : F9 (mouse embryo testicular cancer cell line) whole cell lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 127 kDa
      Observed band size: 127 kDa


      Exposure time: 3 minutes


      Blocking and diluting buffer was 5% NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Basement membrane staining on tumor cells of Mouse stomach tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

      Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Basement membrane staining on tumor cells of Rat stomach tissue is observed.

      Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    Publishing research using ab181551? Please let us know so that we can cite the reference in this datasheet.

    ab181551 has been referenced in 8 publications.

    • Wei L  et al. Expression of integrin a-6 is associated with multi drug resistance and prognosis in ovarian cancer. Oncol Lett 17:3974-3980 (2019). PubMed: 30930993
    • Santos CP  et al. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity. Nat Commun 10:4407 (2019). PubMed: 31562298
    • Xiao YT  et al. Integrin a6-Targeted Positron Emission Tomography Imaging of Colorectal Cancer. ACS Omega 4:15560-15566 (2019). PubMed: 31572857
    • Taschauer A  et al. Peptide-Targeted Polyplexes for Aerosol-Mediated Gene Delivery to CD49f-Overexpressing Tumor Lesions in Lung. Mol Ther Nucleic Acids 18:774-786 (2019). PubMed: 31734558
    • Russo V  et al. Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Sci Rep 8:9690 (2018). PubMed: 29946113
    • Gao X  et al. Physiological stretch induced proliferation of human urothelial cells via integrin a6-FAK signaling pathway. Neurourol Urodyn 37:2114-2120 (2018). PubMed: 29953644
    • Luo L  et al. Gasoline exhaust damages spermatogenesis through downregulating a6-integrin and ß1-integrin in the rat model. Andrologia N/A:e13045 (2018). PubMed: 29781095
    • Kalinina TS  et al. Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro. Mol Biol Rep N/A:N/A (2018). PubMed: 30421125

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human skin (foreskin))
    Permeabilization
    No
    Specification
    Human skin (foreskin)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Unfixed
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human foreskin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris pH 8.4
    Permeabilization
    No
    Specification
    Human foreskin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 31 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha 6 antibody [EPR18124]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Rectum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    No
    Specification
    Rectum
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2016

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