Recombinant
RabMAb

Recombinant Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)

Rabbit recombinant monoclonal Integrin alpha 6 antibody [EPR18124]. Validated in WB, IHC and tested in Mouse, Rat, Human. Cited in 4 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-Integrin alpha 6 antibody [EPR18124]
    See all Integrin alpha 6 primary antibodies
  • Description

    Rabbit monoclonal [EPR18124] to Integrin alpha 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin alpha 6 aa 1-100. The exact sequence is proprietary.
    Database link: P23229

  • Positive control

    • WB: SW480, A549, F6 whole cell lysates; Mouse skin lysate and Rat skin lysate, Human fetal kidney tissue lysate, Mouse kidney and spleen tissue lysate, Rat spleen tissue lysate. IHC-P: Human breast tissue, Human endometrial cancer tissue, Mouse stomach tissue, Rat stomach tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • Tissue specificity

    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • Involvement in disease

    Epidermolysis bullosa letalis, with pyloric atresia
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • Cellular localization

    Cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.  

    Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human endometrial cancer  tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
    Lane 2 : A549 (Human lung carcinoma) whole cell lysate
    Lane 3 : Mouse skin lysate
    Lane 4 : Rat skin lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 127 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM /TBST.

  • Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 127 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM /TBST.

  • All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 127 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer was 5% NFDM /TBST

  • All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution

    Lane 1 : F9 (mouse embryo testicular cancer cell line) whole cell lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 127 kDa
    Observed band size: 127 kDa


    Exposure time: 3 minutes


    Blocking and diluting buffer was 5% NFDM /TBST

  • Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Basement membrane staining on tumor cells of Mouse stomach tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Basement membrane staining on tumor cells of Rat stomach tissue is observed.

    Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Wei L  et al. Expression of integrin a-6 is associated with multi drug resistance and prognosis in ovarian cancer. Oncol Lett 17:3974-3980 (2019). Read more (PubMed: 30930993) »
  • Russo V  et al. Granzyme B is elevated in autoimmune blistering diseases and cleaves key anchoring proteins of the dermal-epidermal junction. Sci Rep 8:9690 (2018). Read more (PubMed: 29946113) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formalin

Abcam user community

Verified customer

Submitted Jun 23 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Rectum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization
No
Specification
Rectum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 30 2016

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