Recombinant Anti-Integrin alpha 6 antibody [EPR18124] (ab181551)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18124] to Integrin alpha 6
- Suitable for: WB, IHC-P, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha 6 antibody [EPR18124]
See all Integrin alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR18124] to Integrin alpha 6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SW480, A549, F6 whole cell lysates; Mouse skin lysate and Rat skin lysate, Human fetal kidney tissue lysate, Mouse kidney and spleen tissue lysate, Rat spleen tissue lysate. IHC-P: Human breast tissue, Human endometrial cancer tissue, Mouse stomach tissue, Rat stomach tissue. IHC-Fr: Rat and mouse stomach tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18124 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).
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IHC-P | (3) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr | (1) |
1/100.
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Notes |
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WB
1/2000. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. |
Target
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Function
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
Involvement in disease
Epidermolysis bullosa letalis, with pyloric atresia -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
Cellular localization
Cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3655 Human
- Entrez Gene: 16403 Mouse
- Entrez Gene: 114517 Rat
- Omim: 147556 Human
- SwissProt: P23229 Human
- SwissProt: Q61739 Mouse
- SwissProt: Q63258 Rat
- Unigene: 133397 Human
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Alternative names
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
Images
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All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ITGA6 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab181551 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab181551 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181551 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Frozen sections) analysis of rat stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.
Positive staining was seen on rat stomach.
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Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on epithelial cells of Human breast tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
Lane 2 : A549 (Human lung carcinoma) whole cell lysate
Lane 3 : Mouse skin lysate
Lane 4 : Rat skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue (4% PFA, 0.2% Tritron x-100) labelling Integrin alpha 6 with ab240252 at 1/100 dilution. ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Negative control using PBS instead of primary antibody (left). Counterstained with DAPI.
Positive staining was seen on mouse stomach.
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Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Membrane and cytoplasm staining on tumor cells of Human endometrial cancer tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution + Human fetal kidney tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
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Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Rat stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/2000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 10 secondsBlocking and diluting buffer was 5% NFDM /TBST
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All lanes : Anti-Integrin alpha 6 antibody [EPR18124] (ab181551) at 1/10000 dilution
Lane 1 : F9 (mouse embryo testicular cancer cell line) whole cell lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 127 kDa
Observed band size: 127 kDa
Exposure time: 3 minutesBlocking and diluting buffer was 5% NFDM /TBST
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Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Intergrin alpha 6 with ab181551 at 1/500 followed by Goat Anti-Rabbit IgG (HRP) at 1/500.
Basement membrane staining on tumor cells of Mouse stomach tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG (HRP) at 1/500.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (32)
ab181551 has been referenced in 32 publications.
- Li W et al. The circ-PITX1 promotes non-small cell lung cancer development via the miR-30e-5p/ITGA6 axis. Cell Cycle 21:304-321 (2022). PubMed: 35007184
- Bélanger C et al. CHARGE syndrome-associated proteins FAM172A and CHD7 influence male sex determination and differentiation through transcriptional and alternative splicing mechanisms. FASEB J 36:e22176 (2022). PubMed: 35129866
- Choi SH et al. KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer. Mol Cancer Res 20:1305-1319 (2022). PubMed: 35394541
- Hao N et al. Laminin-integrin a6b4 interaction activates notch signaling to facilitate bladder cancer development. BMC Cancer 22:558 (2022). PubMed: 35585515
- Karen-Ng LP et al. Extracellular Prostaglandins E1 and E2 and Inflammatory Cytokines Are Regulated by the Senescence Program in Potentially Premalignant Oral Keratinocytes. Cancers (Basel) 14:N/A (2022). PubMed: 35681614