Key features and details
- Rat monoclonal [GoH3] to Integrin alpha 6 (Biotin)
- Suitable for: Flow Cyt
- Reacts with: Mouse, Dog, Human, Cynomolgus monkey
- Conjugation: Biotin
- Isotype: IgG2a
Product nameAnti-Integrin alpha 6 antibody [GoH3] (Biotin)
See all Integrin alpha 6 primary antibodies
DescriptionRat monoclonal [GoH3] to Integrin alpha 6 (Biotin)
Specificityab28080 recognises CD49F (VLA-6 alpha chain)
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse, Dog, Human, Cynomolgus monkey
Tissue/ cell preparation (Mouse) BALB/c mouse mammary tumour cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab28080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIntegrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
Involvement in diseaseEpidermolysis bullosa letalis, with pyloric atresia
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
modificationsIsoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression.
Cellular localizationCell membrane. Cell membrane.
- Information by UniProt
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
ab28080 has been referenced in 3 publications.
- Lim E et al. Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers. Nat Med 15:907-13 (2009). PubMed: 19648928
- Yoshino N et al. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim 49:97-110 (2000). PubMed: 10889948
- Galkowska H et al. Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes. Vet Immunol Immunopathol 53:329-34 (1996). PubMed: 8969052