Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

Thank you for contacting Abcam.

I am sorry that you are having some issues with ab20142well, the only image we have of it being used in IHC-P is from an Abreview and that information can be found below:

https://www.abcam.com/index.html?datasheet=20142&tab=abreviews&intabreviewid=24332

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. To be able to better help in this matter, could you please send me a copy of the protocol that you are using, to see if there is anything I can suggest that will help.

Looking forward to your reply and helping resolve this issue.

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)　 ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney　 ab4287, ab4330, ab4347　 ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus　 ab4287, ab4330, ab4404　 ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617　 ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney　 ab4372, ab4347　 ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus　 ab4287, ab4404　 All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

Thank you for your enquiry regarding our Anti-Integrin a6 Antibodies. The antibodies ab19765 and ab20142 come from different sources and therefore the decision not to test ab19765 in IHC-P comes only from the fact that the laboratory source of this antibody do not perform IHC-P as part of their routine tests. We have no information as to whether it is likely or not to work in IHC-P. I can confirm however that the MP 4F10 clone has been tested in IHC-P and found to not work well in this application, but gave very good results on frozen sections. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.