Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-Integrin alpha 6 antibody [MP 4F10]
See all Integrin alpha 6 primary antibodies -
DescriptionMouse monoclonal [MP 4F10] to Integrin alpha 6
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Host speciesMouse
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Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Cell preparation: SW1222 human colorectal cell line.
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Positive control
- Human lymph node tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferConstituent: 100% PBS
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberMP 4F10
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MyelomaP3-NS1/1-Ag4-1
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IsotypeIgG2b
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Light chain typeunknown
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab20142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. (from PubMed:17370299) | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. (paraformaldehyde or methanol fixed cells) ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionIntegrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
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Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
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Involvement in diseaseEpidermolysis bullosa letalis, with pyloric atresia
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Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
Cellular localizationCell membrane. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 3655 Human
- Omim: 147556 Human
- SwissProt: P23229 Human
- Unigene: 133397 Human
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Alternative names
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
Images
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![Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)](https://www.abcam.com/ps/products/20/ab20142/Images/Integrin-alpha-6-Primary-antibodies-ab20142-2.jpg)
Flow Cytometry - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)Overlay histogram showing HT1080 cells stained with
6 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)](https://www.abcam.com/ps/products/20/ab20142/Images/Integrin-alpha-6-Primary-antibodies-ab20142-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)This image is courtesy of an Anonymous Abreview.ab29871) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/400 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated rabbit anti-mouse polyclonal was used as the secondary antibody, diluted 1/200. -
![Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)](https://www.abcam.com/ps/products/20/ab20142/Images/Integrin-alpha-6-Primary-antibodies-ab20142-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)ICC/IF image of
ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M (ab7481) glycine (ab120050) in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ( ab150113) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Zhang Y et al. CRISPR/Cas9 mediated chicken Stra8 gene knockout and inhibition of male germ cell differentiation. PLoS One 12:e0172207 (2017). Read more (PubMed: 28234938) »
- Kapsokalyvas D et al. Investigating the race for the surface and skin integration in clinically retrieved abutments with two-photon microscopy. Colloids Surf B Biointerfaces 159:97-107 (2017). IHC ; Human . Read more (PubMed: 28780465) »
