Anti-Integrin alpha 6 antibody [MP 4F10] (ab20142)

Thank you for contacting Abcam.

I am sorry that you are having some issues with ab20142well, the only image we have of it being used in IHC-P is from an Abreview and that information can be found below:

https://www.abcam.com/index.html?datasheet=20142&tab=abreviews&intabreviewid=24332

The antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on human samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund. To be able to better help in this matter, could you please send me a copy of the protocol that you are using, to see if there is anything I can suggest that will help.

Looking forward to your reply and helping resolve this issue.

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)　 ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney　 ab4287, ab4330, ab4347　 ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus　 ab4287, ab4330, ab4404　 ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617　 ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney　 ab4372, ab4347　 ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus　 ab4287, ab4404　 All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.