Overview

  • Product name

    Anti-Integrin alpha 9 antibody [EPR9722]
    See all Integrin alpha 9 primary antibodies
  • Description

    Rabbit monoclonal [EPR9722] to Integrin alpha 9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin alpha 9 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • NIH3T3, A673, HepG2, and A549 cell lysates, Human lung and Human skin tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9722
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin.
    • Tissue specificity

      In airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes. Abundantly expressed in fetal lung and lung cancers.
    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha RLC antibody
      • Integrin alpha 4 like antibody
      • Integrin alpha RLC antibody
      • Integrin alpha-9 antibody
      • Integrin alpha-RLC antibody
      • ITA9_HUMAN antibody
      • ITGA 4L antibody
      • ITGA 9 antibody
      • ITGA4L antibody
      • ITGA9 antibody
      • RLC antibody
      see all

    Images

    • All lanes : Anti-Integrin alpha 9 antibody [EPR9722] (ab140599) at 1/1000 dilution

      Lane 1 : NIH3T3 cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 114 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.
    • Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.

    References

    This product has been referenced in:

    • Schips TG  et al. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability. Nat Commun 10:76 (2019). Read more (PubMed: 30622267) »
    • Zhang YL  et al. Integrin a9 Suppresses Hepatocellular Carcinoma Metastasis by Rho GTPase Signaling. J Immunol Res 2018:4602570 (2018). Read more (PubMed: 29951557) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140599.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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