Recombinant
RabMAb

Recombinant Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free (ab240116)

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Overview

  • Product name

    Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free
    See all Integrin alpha 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR9722] to Integrin alpha 9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Integrin alpha 9 aa 50-150. The exact sequence is proprietary.

  • General notes

    Ab240116 is the carrier-free version of ab140599. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240116 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9722

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
WB Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin.

    • Tissue specificity

      In airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes. Abundantly expressed in fetal lung and lung cancers.

    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Alpha RLC antibody
        • Integrin alpha 4 like antibody
        • Integrin alpha RLC antibody
        • Integrin alpha-9 antibody
        • Integrin alpha-RLC antibody
        • ITA9_HUMAN antibody
        • ITGA 4L antibody
        • ITGA 9 antibody
        • ITGA4L antibody
        • ITGA9 antibody
        • RLC antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free (ab240116)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free (ab240116)

        Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140599).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free (ab240116)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] - BSA and Azide free (ab240116)

        Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140599).

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      ab240116 has not yet been referenced specifically in any publications.

      Publishing research using ab240116? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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