Overview

  • Product name

    Anti-Integrin alpha 9+beta 1 antibody [Y9A2]
  • Description

    Mouse monoclonal [Y9A2] to Integrin alpha 9+beta 1
  • Host species

    Mouse
  • Specificity

    Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-FoFr,IHC-P or IHC-R
  • Species reactivity

    Reacts with: Rat, Guinea pig, Human
  • Immunogen

    Tissue/ cell preparation (Human): alpha9 integrin transfected lambda cells.

  • General notes


    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Ab27947 was purified by affinity chromatography, from tissue culture supernatant.
  • Primary antibody notes

    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.
  • Clonality

    Monoclonal
  • Clone number

    Y9A2
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20810886
Flow Cyt Use 10µl for 106 cells.

(in 100µl). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-FoFr,IHC-P or IHC-R.
  • Target

    • Relevance

      Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Integrin alpha 9, when bound to the integrin beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of integrin alpha 9 has been found to be upregulated in small cell lung cancers.
    • Cellular localization

      Type I membrane protein.
    • Database links

    • Alternative names

      • CD29 antibody
      • FNRB antibody
      • GPIIA antibody
      • ITGA9 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    References

    This product has been referenced in:

    See all 8 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you for your enquiry.

    The following information is taken from Human Protein Atlas.

    1) Integrin alpha 9 positive control: Digestive tract
    http://www.proteinatlas.org/ENSG00000144668

    2) Integrin beta 1 positive control: Digestive tract , liver, pancreas, small intestine
    http://www.proteinatlas.org/ENSG00000150093

    Also, SwissProt (Tissue specificity) section lists some other possible tissue types that can also be used as positive controls. Please refer to the following links.

    http://www.uniprot.org/uniprot/Q13797
    http://www.uniprot.org/uniprot/P05556

    I hope the information will be helpful.

    Read More
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Endothelial cells and fibroblasts cultured from hu)
    Specification
    Endothelial cells and fibroblasts cultured from hu
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up