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Anti-Integrin alpha V + beta 6 antibody [avß6 53a.2] (ab97588)

Reviews (2)Q&A (3)References (6)

Key features and details

  • Rat monoclonal [avß6 53a.2] to Integrin alpha V + beta 6
  • Suitable for: ICC/IF, Neutralising, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Integrin alpha V antibody [EPR19669] (ab208012)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Integrin alpha V + beta 6 antibody [avß6 53a.2]
    See all Integrin alpha V + beta 6 primary antibodies

  • Description

    Rat monoclonal [avß6 53a.2] to Integrin alpha V + beta 6

  • Host species

    Rat

  • Tested applications

    Suitable for: ICC/IF, Neutralising, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein corresponding to Integrin alpha V + beta 6. Mouse fibroblasts retrovirally infected with cDNA to human beta6 (3T3b6.19). Cells expressed a mouse/human avb6 chimera.
    Database link: P06756

  • Epitope

    Extracelllular region of the heterodimer.

  • Positive control

    • BT20 breast carcinoma, original immunogen.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
Use at an assay dependent concentration.
Neutralising
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD51 antibody
      • Integrin alpha-V light chain antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • Integrin, alpha V antibody
      • Integrin, beta 6 antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • ITGB6 antibody
      • Vitronectin receptor subunit alpha antibody
      see all

    References (6)

    Publishing research using ab97588? Please let us know so that we can cite the reference in this datasheet.

    ab97588 has been referenced in 6 publications.

    • Schiesser JV  et al. Integrin avß5 heterodimer is a specific marker of human pancreatic beta cells. Sci Rep 11:8315 (2021). PubMed: 33859325
    • Ran R  et al. A role for CBFß in maintaining the metastatic phenotype of breast cancer cells. Oncogene 39:2624-2637 (2020). PubMed: 32005976
    • Liu H  et al. IL-32? promotes integrin avß6 expression through the activation of NF-?B in HSCs. Exp Ther Med 14:3880-3886 (2017). PubMed: 29042996
    • Coughlan L  et al. Combined fiber modifications both to target a(v)ß(6) and detarget the coxsackievirus-adenovirus receptor improve virus toxicity profiles in vivo but fail to improve antitumoral efficacy relative to adenovirus serotype 5. Hum Gene Ther 23:960-79 (2012). Flow Cyt ; Human . PubMed: 22708837
    • Marsh D  et al. alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation. Cancer Res 68:3295-303 (2008). PubMed: 18451156
    • Myou S  et al. Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2-integrin caused by ERK-independent activation of cPLA2. Immunology 107:77-85 (2002). PubMed: 12225365

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Integrin alpha V + beta 6 antibody [avß6 53a.2]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Embryonic Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    Yes - PBS + 0.1% Triton
    Specification
    Embryonic Pancreas
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    MS. Heather Wilson

    Verified customer

    Submitted Mar 08 2021

    Flow Cytometry abreview for Anti-Integrin alpha V + beta 6 antibody [avß6 53a.2]

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (BT-20 Breast carcinoma cells)
    Specification
    BT-20 Breast carcinoma cells
    Preparation
    Cell harvesting/tissue preparation method: Trypsinised from cell culture flask
    Sample buffer: serum free DMEM medium
    Fixation
    Unfixed
    Permeabilization
    No
    Gating Strategy
    Live cell population
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2012

    Question

    Thank you for your kind email. I would be happy to test the antibody as you have proposed. Please send me the discount code so that I can go ahead with the purchase order. I am looking forward to seeing how the antibody performs (hoping of course that it works really well for the application that I need it for – we shall see).

    Many Thanks.

    Read More
    Answer

    Thank you for your reply.

    I am very pleased to hear you would like to accept our offer and test ab97588 in IHC-Fr. I have arranged the following code for you:

    DISCOUNT CODE: 100%ABR-E76Z3
    Expiration date: 11/11/12

    This code will give you: 1 freeprimary antibodybefore the expiration date.

    To redeem this offer, please submit an Abreview forIHC-Fr and include this code in the 'Additional Comments' section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Would you be kind enough to offer/send me a tiny aliquot of this Antibody to test it for IHC-frozen?

    Kindest Regards,

    Read More
    Answer

    Thank you for your reply.

    I am sorry to confirm that because we carry over 80,000 products, it is not feasible for us to keep small sample sizes of our products.

    As ab97588 has not yetbeen tested in IHC-Fr, I can offer a discount off a future purchase if you buy ab97588 now, test it inIHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab97588 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab97588 so please wait for my reply before ordering.

    2. Purchase ab97588 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab97588 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Dear Abcam,

    Does the antibody below work in IHC on frozen sections (I know the datasheet says that it works in IF, but does it with IHC-fr)? If so, please can you indicate protocol that was specifically used.

    Anti-Integrin alpha V + beta 6 antibody [avß6 53a.2] (ab97588)

    Many Thanks.

    Read More
    Answer

    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab7588 has never been tested in frozen sections, IHC-Fr. All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. It has been tested in ICC-IF which is florescent immunocytochemistry, which is staining of cell culture.

    If you would like to test the antibody in IHC-Fr, please do not hesitate to contact me again prior to the purchase by replying to this message as you may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

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    For licensing inquiries, please contact partnerships@abcam.com