Product nameAnti-Integrin alpha V antibody [272-17E6]
See all Integrin alpha V primary antibodies
DescriptionMouse monoclonal [272-17E6] to Integrin alpha V
SpecificityCross reacts with all alpha V integrins.
Tested applicationsSuitable for: Inhibition Assay, ICC/IF, Blocking, Flow Cyt, ELISA, IPmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Full length native protein (purified) (Human).
- Human breast carcinoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab16821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration. PubMed: 19115199|
|Flow Cyt||Use a concentration of 2.5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|IP||Use a concentration of 2.5 µg/ml.|
FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
ab16821 staining Integrin alpha V in Human prostate cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/250 in 1% BSA/PBS) for 15 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as secondary antibody.
ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.
This product has been referenced in:
- Tong MH et al. Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication. Sci Rep 7:12402 (2017). ICC/IF ; Human . Read more (PubMed: 28963517) »
- Miklos W et al. Loss of phosphodiesterase 4D mediates acquired triapine resistance via Epac-Rap1-Integrin signaling. Oncotarget 7:84556-84574 (2016). Read more (PubMed: 27602951) »