Overview

  • Product name
    Anti-Integrin alpha V antibody [272-17E6]
    See all Integrin alpha V primary antibodies
  • Description
    Mouse monoclonal [272-17E6] to Integrin alpha V
  • Host species
    Mouse
  • Specificity
    Cross reacts with all alpha V integrins.
  • Tested applications
    Suitable for: Inhibition Assay, ICC/IF, Blocking, Flow Cyt, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • Human breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab16821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 19115199
Flow Cyt Use a concentration of 2.5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
IP Use a concentration of 2.5 µg/ml.

Target

  • Function
    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • Integrin alpha five antibody
    • integrin alpha V beta 3 antibody
    • Integrin alpha-5 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Images

  • ab16821 staining Integrin alpha V in Human prostate cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/250 in 1% BSA/PBS) for 15 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Tong MH  et al. Preferential sensing and response to microenvironment stiffness of human dermal fibroblast cultured on protein micropatterns fabricated by 3D multiphoton biofabrication. Sci Rep 7:12402 (2017). ICC/IF ; Human . Read more (PubMed: 28963517) »
  • Miklos W  et al. Loss of phosphodiesterase 4D mediates acquired triapine resistance via Epac-Rap1-Integrin signaling. Oncotarget 7:84556-84574 (2016). Read more (PubMed: 27602951) »
See all 15 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Question
Answer

Vielen Dank für Ihre Anfrage.

Das Labor, von welchem wir diesen Antikörper beziehen, hat uns tatsächlich informiert, dass dieser Antikörper nicht auf Paraffinschnitten funktionieren sollte. Dies ist eine Information von dem Labor, welches den Antikörper ursprünglich hergestellt hat.

Ich entschuldige mich für diese Umstände, und danke Ihnen, unsere Aufmerksamkeit auf dies gelenkt zu haben. Ich habe das Datenblatt aktualisiert und betroffene Kunden werden informiert.

Kann ich Ihnen vielleicht andersweitig helfen? Wir haben zwei polyklonale Antikörper, welche auf Paraffinschnitten validiert wurden: ab76609 und ab117611.
https://www.abcam.com/index.html?datasheet=76609 (or use the following: https://www.abcam.com/index.html?datasheet=76609).
https://www.abcam.com/index.html?datasheet=117611 (or use the following: https://www.abcam.com/index.html?datasheet=117611).

Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen oder Bedenken haben. Ich wünsche Ihnen ein schönes Wochenende.

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Answer

The exact target epitope was not characterized in-house. Please see the references listed above for more details.
We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse samples.

I would like to encourage you to take advantage of our Abreview program to leave reviews for any Abcam product. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. Reviews are provided by customers and may be good or bad. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and click Submit an Abreview. You can gain more Abpoints by submitting images as well.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

Therefore, we would be pleased to provide a replacement from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and you prefer a credit note in this case, I will be pleased to arrange this for you.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

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Answer

I am sorry about the issues you have been having. Thank you for bringing this to our attention.

I would very much like to help you with this. However to do that I need the particulars of your protocol. This information is important not only in helping us understand the problems that you are experiencing but in our internal testing as well.

Could you tell me the tissue types and lysis buffer that you have used?

Have you reduced and denatured your samples? Which method/buffers did you use?

How much total protein did you use per well?

Did you load a loading control? Which LC did you use? What were the results?

Did you load a positive control? Which PC did you use? What were the results?

What membrane type did you transfer to?

What blocking steps did you use?

What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

Have you run a no primary antibody control blot? What were the results?

What is the wash buffer that you use?

Could you provide the Abcam order confirmation number or PO number used to purchase this?

Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.

Read More

Answer

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)  ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney  ab4287, ab4330, ab4347  ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus  ab4287, ab4330, ab4404  ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617  ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney  ab4372, ab4347  ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus  ab4287, ab4404  All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (melanoma cell line)
Specification
melanoma cell line
Preparation
Cell harvesting/tissue preparation method: cell line - trypsinized cells, treated with STI and spun out trypsin, kept on ice during experiment duration
Sample buffer: 2% FCS/0.1%NaAzide/PBS
Fixation
none
Permeabilization
No
Gating Strategy
FSC and SSC for healthy cell population, excluded PI positive cells

Abcam user community

Verified customer

Submitted Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (prostate)
Specification
prostate
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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