Key features and details
- Rabbit polyclonal to Integrin alpha V - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Integrin alpha V antibody - C-terminal
See all Integrin alpha V primary antibodies
DescriptionRabbit polyclonal to Integrin alpha V - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig
Recombinant fragment, corresponding to a region within C terminal amino acids 722-944 of Human Integrin alpha V (UniProt: P06756).
- Recombinant Human Integrin alpha V protein (Tagged) (ab114240) can be used as a positive control in WB. RD (Rhabdomyosarcoma) cells; RD cells transfected with scramble siRNA; HUVEC whole cell lysate; Human colon carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 116 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
All lanes : Anti-Integrin alpha V antibody - C-terminal (ab154384) at 1/900 dilution
Lane 1 : RD (Rhabdomyosarcoma) whole cell lysate (Mock control)
Lane 2 : RD transfected scramble siRNA whole cell lysate
Lane 3 : RD transfected Integrin alpha V siRNA whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 116 kDa
8% SDS PAGE
Anti-Integrin alpha V antibody - C-terminal (ab154384) at 1/500 dilution + HUVEC whole cell lysate at 30 µg
Predicted band size: 116 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Integrin alpha V with ab154384 at 1/250 dilution.
ab154384 has not yet been referenced specifically in any publications.