Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse skin)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
Mouse skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in 1X TBST
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG(H+L)-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
25 second(s)
Bands
Specific: 125 kDa Non-specific: 60, 75 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 20 2018