Recombinant Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Integrin alpha V antibody [EPR16800] (HRP)
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR16800] to Integrin alpha V (HRP) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
Database link: P06756 -
Positive control
- WB: Human Fetal Brain tissue lysate. IHC-P: normal human kidney tissue sections
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16800 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Integrin alpha V antibody [EPR16800] (ab179475)
- Anti-Integrin alpha V antibody [EPR16800] (Alexa Fluor® 647) (ab204684)
- Anti-Integrin alpha V antibody [EPR16800] (Alexa Fluor® 594) (ab207286)
- Anti-Integrin alpha V antibody [EPR16800] (Phycoerythrin) (ab211845)
- Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 116 kDa). |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474)](https://www.abcam.com/ps/products/205/ab205474/Images/ab205474-286945-IHCAP00233711.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474) -
![Western blot - Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474)](https://www.abcam.com/ps/products/205/ab205474/Images/ab205474-270791-WBab2054741.jpg)
Western blot - Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474)Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474) at 1/2000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205474 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
References
ab205474 has not yet been referenced specifically in any publications.
