Recombinant
RabMAb

Recombinant Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474)

Overview

  • Product name

    Anti-Integrin alpha V antibody [EPR16800] (HRP)
    See all Integrin alpha V primary antibodies
  • Description

    Rabbit monoclonal [EPR16800] to Integrin alpha V (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
    Database link: P06756

  • Positive control

    • WB: Human Fetal Brain tissue lysate. IHC-P: normal human kidney tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab205474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 116 kDa).

Target

  • Function

    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • Integrin alpha five antibody
    • integrin alpha V beta 3 antibody
    • Integrin alpha-5 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Images

  • Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474) at 1/2000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 116 kDa
    Observed band size: 135 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205474 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab205474 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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