Product nameAnti-Integrin alpha V antibody [EPR16800] (HRP)
See all Integrin alpha V primary antibodies
DescriptionRabbit monoclonal [EPR16800] to Integrin alpha V (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
Database link: P06756
- WB: Human Fetal Brain tissue lysate. IHC-P: normal human kidney tissue sections
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Integrin alpha V antibody [EPR16800] (ab179475)
- Anti-Integrin alpha V antibody [EPR16800] (Alexa Fluor® 647) (ab204684)
- Anti-Integrin alpha V antibody [EPR16800] (Alexa Fluor® 594) (ab207286)
- Anti-Integrin alpha V antibody [EPR16800] (Phycoerythrin) (ab211845)
- Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
Our Abpromise guarantee covers the use of ab205474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000. Detects a band of approximately 135 kDa (predicted molecular weight: 116 kDa).|
FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
Anti-Integrin alpha V antibody [EPR16800] (HRP) (ab205474) at 1/2000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 116 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205474 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205474 has not yet been referenced specifically in any publications.