Product nameAnti-Integrin alpha V antibody [EPR5583]
See all Integrin alpha V primary antibodies
DescriptionRabbit monoclonal [EPR5583] to Integrin alpha V
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Integrin alpha V aa 1000-1100 (intracellular). The exact sequence is proprietary.
Database link: P06756
- A549, A431, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; A549 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (KD)KD = 3.20 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
All lanes : Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution
Lane 1 : A549 cell lysates
Lane 2 : A431 cell lysates
Lane 3 : C6 cell lysates
Lane 4 : RAW264.7 cell lysates
Lane 5 : PC12 cell lysates
Lane 6 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 86, 116 kDa
ab124968 at 1/10 dilution staining Integrin alpha V in permeabilized A549 cells by Flow cytometry (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Mygind KJ et al. Loss of ADAM9 expression impairs ß1 integrin endocytosis, focal adhesion formation and cancer cell migration. J Cell Sci 131:N/A (2018). Read more (PubMed: 29142101) »
- Winkler J et al. Cellular apoptosis susceptibility (CAS) is linked to integrin ß1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget 7:22883-92 (2016). WB . Read more (PubMed: 27015362) »