Anti-Integrin alpha V antibody [EPR5583] (ab124968)


  • Product name
    Anti-Integrin alpha V antibody [EPR5583]
    See all Integrin alpha V primary antibodies
  • Description
    Rabbit monoclonal [EPR5583] to Integrin alpha V
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Integrin alpha V aa 1000-1100 (intracellular). The exact sequence is proprietary.
    Database link: P06756

  • Positive control
    • A549, A431, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; A549 cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD51 antibody
      • DKFZp686A08142 antibody
      • Integrin alpha five antibody
      • integrin alpha V beta 3 antibody
      • Integrin alpha-5 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • integrin alphaVbeta3 antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • MSK 8 antibody
      • Msk8 antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      • VTNR antibody
      see all


    • All lanes : Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : A431 cell lysates
      Lane 3 : C6 cell lysates
      Lane 4 : RAW264.7 cell lysates
      Lane 5 : PC12 cell lysates
      Lane 6 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 86, 116 kDa

    • ab124968 at 1/10 dilution staining Integrin alpha V in permeabilized A549 cells by Flow cytometry (red) or a rabbit IgG (negative) (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Winkler J  et al. Cellular apoptosis susceptibility (CAS) is linked to integrin ß1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget 7:22883-92 (2016). WB . Read more (PubMed: 27015362) »
    • Zhang C  et al. Molecular magnetic resonance imaging of activated hepatic stellate cells with ultrasmall superparamagnetic iron oxide targeting integrin avß3 for staging liver fibrosis in rat model. Int J Nanomedicine 11:1097-108 (2016). WB . Read more (PubMed: 27051285) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    The higher MW band (˜ 150 KDa) on the blot for ab124968 is full length Integrin alpha-V, and the lower band (˜ 25 KDa) is Integrin alpha-V light chain ( At this time we have not run any experiments to characterize...

    Read More


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