Recombinant Anti-Integrin alpha V antibody [EPR5583] (ab124968)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5583] to Integrin alpha V
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha V antibody [EPR5583]
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR5583] to Integrin alpha V -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Integrin alpha V aa 1000-1100 (intracellular). The exact sequence is proprietary.
Database link: P06756 -
Positive control
- A549, A431, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 3.20 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5583 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa). |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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All lanes : Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86, 116 kDa
Observed band size: 150, 27-30 kDa why is the actual band size different from the predicted?The bands detected are consistent with what have been described in the literatures (PMID: 11741954, 27033701). Full-length Integrin alpha V: 150kDa; Integrin alpha V light chain: 27-30kDa
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab124968 has been referenced in 9 publications.
- Ma M et al. p53 positively regulates the proliferation of hepatic progenitor cells promoted by laminin-521. Signal Transduct Target Ther 7:290 (2022). PubMed: 36042225
- Nguyen SL et al. Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo. Sci Rep 11:4217 (2021). PubMed: 33602965
- McRae MP et al. Nuclear F-actin Cytology in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma. J Dent Res 100:479-486 (2021). PubMed: 33179547
- Aseervatham J & Ogbureke KUE Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630820
- Kamarajan P et al. Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin. PLoS Pathog 16:e1008881 (2020). PubMed: 33002094
- Mygind KJ et al. Loss of ADAM9 expression impairs ß1 integrin endocytosis, focal adhesion formation and cancer cell migration. J Cell Sci 131:N/A (2018). PubMed: 29142101
- Barr S et al. Development, fabrication and evaluation of a novel biomimetic human breast tissue derived breast implant surface. Acta Biomater 49:260-271 (2017). PubMed: 27919840
- Winkler J et al. Cellular apoptosis susceptibility (CAS) is linked to integrin ß1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget 7:22883-92 (2016). WB . PubMed: 27015362
- Zhang C et al. Molecular magnetic resonance imaging of activated hepatic stellate cells with ultrasmall superparamagnetic iron oxide targeting integrin avß3 for staging liver fibrosis in rat model. Int J Nanomedicine 11:1097-108 (2016). WB . PubMed: 27051285