Key features and details
- Mouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3
- Suitable for: Flow Cyt, IP, IHC-Fr, Blocking
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]
See all Integrin alpha V beta 3 primary antibodies
DescriptionMouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3
SpecificityReacts with the Integrin alpha V beta 3
Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, Blockingmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
The details of the immunogen for this antibody are not available.
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS
Concentration information loading...
PurityTissue culture supernatant
Purification notesPurified from TCS.
Clone number27.1 (VNR-1)
Our Abpromise guarantee covers the use of ab78289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. 4-10% formalin fixed frozen tissues (short fixation times, 10 minutes room temperature) only'|
|Blocking||Use at an assay dependent concentration. Competes with clone LM609 for integrin function blocking.|
RelevanceFunction: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
Cellular localizationCell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
- Antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD 61 antibody
Overlay histogram showing A431 cells stained with ab78289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78289, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
ab78289 has been referenced in 23 publications.
- Lambshead JW et al. Long-Term Maintenance of Human Pluripotent Stem Cells on cRGDfK-Presenting Synthetic Surfaces. Sci Rep 8:701 (2018). PubMed: 29335618
- Vogt D et al. Premacular membranes in tissue culture. Graefes Arch Clin Exp Ophthalmol N/A:N/A (2018). PubMed: 29931427
- Yang WY et al. Association of aquaporin-1 with tumor migration, invasion and vasculogenic mimicry in glioblastoma multiforme. Mol Med Rep 17:3206-3211 (2018). PubMed: 29257313
- Wen Z et al. Antitumor activity of recombinant RGD-IFN-a2a-core fusion protein in vitro. Anticancer Drugs 28:31-39 (2017). Flow Cyt ; Human . PubMed: 27759573
- Freeman R et al. Instructing cells with programmable peptide DNA hybrids. Nat Commun 8:15982 (2017). PubMed: 28691701