Overview

  • Product name

    Anti-Integrin alpha V beta 3 antibody [27.1 (VNR-1)]
    See all Integrin alpha V beta 3 primary antibodies
  • Description

    Mouse monoclonal [27.1 (VNR-1)] to Integrin alpha V beta 3
  • Host species

    Mouse
  • Specificity

    Reacts with the Integrin alpha V beta 3
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, IHC-Fr, Blockingmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab78289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22655084
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. 4-10% formalin fixed frozen tissues (short fixation times, 10 minutes room temperature) only'
Blocking Use at an assay dependent concentration. Competes with clone LM609 for integrin function blocking.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance

      Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
    • Cellular localization

      Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
    • Database links

    • Alternative names

      • Antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD 61 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • Integrin beta 3 antibody
      • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
      • Integrin beta chain beta 3 antibody
      • integrinVbeta3 antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • MSK 8 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor alpha polypeptide antibody
      • vitronectin receptor antibody
      • Vitronectin receptor subunit alpha antibody
      • VNR 1 antibody
      • VNR1 antibody
      • VNRA antibody
      see all

    Images

    • Immunofluorescence analysis of U87MG cells, staining Integrin alpha V beta 3 (red) with ab78289 at 1/500 dilution.

      Cells were either untreated (left) or treated with 10 nM PTX for 12 hours (right). Nuclei were stained with DAPI (blue).

      This image was generated using the ascites version of the product.

    • Overlay histogram showing A431 cells stained with ab78289 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78289, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    References

    This product has been referenced in:

    See all 21 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (MDA-MB-231)
    Permeabilization
    No
    Specification
    MDA-MB-231
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 24 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (TIME cells)
    Gel Running Conditions
    Reduced Denaturing (polyacrylamide 10% gel)
    Loading amount
    15 µg
    Treatment
    5 ng/ml VEGF for 15 or 60 minutes in low or high glucose medium
    Specification
    TIME cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jan 24 2018

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Candida albicans Cell (Hypha Cell)
    Permeabilization
    Yes - Saponin
    Specification
    Hypha Cell
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Angela Lopez

    Verified customer

    Submitted Jun 15 2016

    Answer

    Thank you for contacting us.

    The hybridoma was generated from B cell isolated from a mouse immunized with alphaVbeta3, the monoclonal produced from the hybridoma recoginzes a conformational epitope. We do not know whether this monoclonal recognizes hamster VNR1 or not. The only way to find out is to test it directly. Blast search is not possible since the antibody was not raised against a linear peptide.

    If you wish to test this antibody in hamster, we do have a testing discount program that gives you a discount off a future purchase in exchange for a review on the tested species on our website.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)  ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney  ab4287, ab4330, ab4347  ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus  ab4287, ab4330, ab4404  ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617  ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)  ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney  ab4372, ab4347  ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus  ab4287, ab4404  All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

    Read More

    Answer

    Thank you for contacting us. I received the following information from the lab: The antibody was tested for use in IHC by external academic laboratories. The researchers used human heart artery segments, that were snap frozen in OCT. Other positive controls that can be used are: human kidney tissue, A-431 cell line. All positive tissues and cell lines come from humans. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Embryonic Fibroblast (MEF))
    Specification
    Mouse Embryonic Fibroblast (MEF)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton

    Abcam user community

    Verified customer

    Submitted Sep 16 2011

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cell line ( cervical cancer))
    Specification
    HeLa cell line ( cervical cancer)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton

    Abcam user community

    Verified customer

    Submitted Sep 05 2011

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